Chen Yan, Wang Yuqing, Yang Caiting, Zhang Xiaoting, Liu Yongxin, Pu Genyuan, Jiang Huijie, Pan Yun, Li Zhenjin, Lai Mingming
Dali University, Dali, 671000, China.
Dali University Affiliated Hospital, Dali, 671000, China.
Heliyon. 2023 Aug 26;9(9):e19540. doi: 10.1016/j.heliyon.2023.e19540. eCollection 2023 Sep.
FOXG1, a transcriptional factor belonging to the Forkhead Box (Fox) superfamily, is highly expressed in the brain tissue during brain development and plays an important role in cellular proliferation. Recently, FOXG1 was reported to play important roles in oncogenesis, wherein its abnormal expression regulates tumor cell proliferation. However, the expression and role of FOXG1 in lung cancer remain largely unknown. This study investigated the clinical significance, expression, and role of FOXG1 in lung cancer. We found that FOXG1 was highly expressed in lung cancer tissues. MTT, CCK-8 and colony formation assays showed that FOXG1 overexpression could enhance the proliferation of A549 lung cancer cells. Flow cytometry analysis revealed that FOXG1 promoted the cell cycle and suppressed cell apoptosis. Additionally, the expression levels of PTEN, phosphorylated AKT, mTOR, p53, and Bax were significantly altered in response to changes in FOXG1 expression, indicating that FOXG1 regulated the PI3K pathway. Furthermore, in the xenograft mouse model, the upregulated FOXG1 expression strongly promoted tumor growth. In conclusion, these results suggested that FOXG1 was a critical regulator of the proliferation of lung cancer cells and enhanced tumor growth .
叉头框蛋白G1（FOXG1）是属于叉头框（Fox）超家族的一种转录因子，在脑发育过程中于脑组织中高度表达，并在细胞增殖中发挥重要作用。最近，有报道称FOXG1在肿瘤发生中起重要作用，其异常表达可调节肿瘤细胞增殖。然而，FOXG1在肺癌中的表达及作用仍 largely未知。本研究调查了FOXG1在肺癌中的临床意义、表达及作用。我们发现FOXG1在肺癌组织中高表达。MTT、CCK - 8和集落形成试验表明，FOXG1过表达可增强A549肺癌细胞的增殖。流式细胞术分析显示，FOXG1促进细胞周期进程并抑制细胞凋亡。此外，随着FOXG1表达的变化，PTEN、磷酸化AKT、mTOR、p53和Bax的表达水平发生显著改变，表明FOXG1调节PI3K通路。此外，在异种移植小鼠模型中，上调的FOXG1表达强烈促进肿瘤生长。总之，这些结果表明FOXG1是肺癌细胞增殖的关键调节因子，并促进肿瘤生长。
