Kiros Teklehaimanot, Belete Debaka, Andualem Tesfaye, Workineh Lemma, Tilahun Mekdes, Eyayu Tahir, Getie Birhanu, Tiruneh Tegenaw, Kiflom Saymon, Damtie Shewaneh, Gebreyesus Tsehaynesh
Department of Medical Laboratory Sciences, College of Health Sciences and School of Medicine, Debre Tabor University, Debre Tabor, Ethiopia.
Department of Medical Microbiology, School of Biomedical and Laboratory Sciences, College of Medicine and Health Sciences, University of Gondar, Gondar, Ethiopia.
Heliyon. 2023 Sep 12;9(9):e20072. doi: 10.1016/j.heliyon.2023.e20072. eCollection 2023 Sep.
Antimicrobial resistance has remained global public health threat. Carriage with drug-resistant bacterial pathogens, particularly beta-lactamase and carbapenemase-producing is among the most concerning. The purpose of this study was to look into the magnitude, antimicrobial resistance patterns, and associated risk factors among hospitalized patients.
A facility-based cross-sectional study was conducted on 383 hospitalized patients at Debre Tabor Comprehensive Specialized Hospital between September 2022 and May 2023. A pre-tested structured questionnaire was used to collect sociodemographic and clinical data. The data on the etiologic agent was collected using standard bacteriological techniques. Briefly, stool specimens were collected aseptically into sterile, leak-proof stool cups. The stool sample was inoculated onto MacConkey agar and incubated aerobically at 37 °C for 24 h. The species isolation and antimicrobial resistance patterns were then performed adhering to bacteriological procedures. In the analysis, a p-value of <0.05 was considered statistically significant.
There were 383 study participants, and men made up the majority (55.6%). The study participants' mean age was 33 ± 18 years. Three hundred and seventy-seven (88%) of the study's participants had no previous history of antibiotic use. There were 102 (26.6%) and 21 (5.5%) cases of gastrointestinal carriage caused by that produce beta-lactamase and carbapenemase, respectively. In total, 175 isolates of were detected. (n = 89) and (n = 51) were the most frequently recovered. In this study, 46 (79.3%) and 8 (13.8%) isolates of that produce beta-lactamase were resistant to ampicillin and amoxicillin/clavulanic acid, respectively. Furthermore, participants who had previously used antibiotics experienced a two-fold increase in exposure to gastrointestinal tract carriage by carbapenemase-producing [AOR, 95% CI (2.01, 1.06-2.98), p = 0.001].
The emergence of drug-resistant pathogens is a growing concern. An increase in the prevalence of drug-resistant infections in hospitalized patients is warranting further investigation.
抗菌药物耐药性仍然是全球公共卫生威胁。携带耐药性细菌病原体，尤其是产β-内酰胺酶和碳青霉烯酶的病原体，是最令人担忧的问题之一。本研究的目的是调查住院患者中耐药性的严重程度、抗菌药物耐药模式及相关危险因素。
2022年9月至2023年5月期间，在德布雷塔博尔综合专科医院对383名住院患者进行了一项基于机构的横断面研究。使用预先测试的结构化问卷收集社会人口学和临床数据。使用标准细菌学技术收集病原体数据。简要地说，粪便标本无菌采集到无菌、防漏的粪便杯中。粪便样本接种到麦康凯琼脂上，在37°C有氧条件下培养24小时。然后按照细菌学程序进行菌种分离和抗菌药物耐药模式分析。在分析中，p值<0.05被认为具有统计学意义。
共有383名研究参与者，其中男性占大多数（55.6%）。研究参与者的平均年龄为33±18岁。377名（88%）研究参与者既往无抗生素使用史。分别有102例（26.6%）和21例（5.5%）胃肠道携带产β-内酰胺酶和碳青霉烯酶的病原体。总共检测到175株病原体。大肠埃希菌（n = 89）和肺炎克雷伯菌（n = 51）是最常分离出的菌株。在本研究中，产β-内酰胺酶的大肠埃希菌分离株中有46株（79.3%）对氨苄西林耐药，8株（13.8%）对阿莫西林/克拉维酸耐药。此外，既往使用过抗生素的参与者感染产碳青霉烯酶病原体的胃肠道携带风险增加了两倍[AOR，95%CI（2.01，1.06 - 2.98），p = 0.001]。
耐药病原体的出现日益受到关注。住院患者中耐药感染患病率的增加值得进一步调查。
