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一种用于测定线虫个体携带现象发生率的简便且低成本的方法。

An easy and inexpensive method for determining the rate of individual phoretic events of nematodes.

作者信息

Williams Benjamin, Nelson Matthew, McRobert Scott, Fingerut Jonathan

机构信息

Biology, Saint Joseph's University, Philadelphia, Pennsylvania, United States.

出版信息

MicroPubl Biol. 2023 Sep 21;2023. doi: 10.17912/micropub.biology.000942. eCollection 2023.

Abstract

Considering their limited locomotory capabilities, the cosmopolitan distribution of free-living nematodes may rely on phoretic dispersal. We describe a new, inexpensive device to investigate individual phoretic events of the nematode using the pomace flies and over short time periods. Using our device, we replicated previous findings demonstrating that phoresis requires to be in the dauer stage and capable of nictation. Additionally, we find that phoresis can happen on the order of seconds, and does not increase linearly with time of interaction. Using this approach can facilitate the investigation of nematode biogeography, which could provide useful insight into their, and their vector's, control.

摘要

考虑到自由生活线虫有限的移动能力,其在全球的分布可能依赖于携带传播。我们描述了一种新型廉价装置,用于在短时间内研究线虫借助果蝇进行的个体携带传播事件。利用我们的装置,我们重复了之前的发现,即携带传播需要线虫处于 dauer 阶段且具备点头行为能力。此外,我们发现携带传播能在数秒内发生,且不会随相互作用时间线性增加。采用这种方法有助于对线虫生物地理学进行研究,这可为线虫及其传播媒介的控制提供有用的见解。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5480/10556881/6df9b88ca2c7/25789430-2023-micropub.biology.000942.jpg

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