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基于逆转录重组酶辅助扩增技术的两种检测方法的建立，用于快速检测 H5 亚型禽流感病毒。

Establishment of two assays based on reverse transcription recombinase-aided amplification technology for rapid detection of H5 subtype avian influenza virus.

机构信息

China Animal Health and Epidemiology Center , Qingdao, China.

School of Life Science and Food Engineering, Hebei University of Engineering , Hebei, China.

出版信息

Microbiol Spectr. 2023 Dec 12;11(6):e0218623. doi: 10.1128/spectrum.02186-23. Epub 2023 Oct 9.

DOI:10.1128/spectrum.02186-23
PMID:37811963
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10715165/
Abstract

Avian influenza virus (AIV) subtype H5 is a highly contagious zoonotic disease and a serious threat to the farming industry and public health. Traditional detection methods, including virus isolation and real-time PCR, require tertiary biological laboratories and are time-consuming and complex to perform, making it difficult to rapidly diagnose H5 subtype avian influenza viruses. In this study, we successfully developed two methods, namely, RF-RT-RAA and RT-RAA-LFD, for rapid detection of H5-AIV. The assays are characterized by their high specificity, sensitivity, and user-friendliness. Moreover, the results of the reaction can be visually assessed, which are suitable for both laboratory testing and grassroots farm screening for H5-AIV.

摘要

禽流感病毒（AIV）亚型 H5 是一种高传染性的人畜共患病，对农业产业和公共卫生构成严重威胁。传统的检测方法，包括病毒分离和实时 PCR，需要三级生物实验室，并且操作繁琐复杂，难以快速诊断 H5 亚型禽流感病毒。在本研究中，我们成功开发了两种方法，即 RF-RT-RAA 和 RT-RAA-LFD，用于快速检测 H5-AIV。这些检测方法具有高度的特异性、灵敏度和易用性。此外，反应结果可以进行直观评估，适用于实验室检测和基层农场的 H5-AIV 筛查。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ec45/10715165/cbca90b38a37/spectrum.02186-23.f008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ec45/10715165/ab71205d95f4/spectrum.02186-23.f001.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ec45/10715165/f94fd59b1e0d/spectrum.02186-23.f003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ec45/10715165/41ad3bb904f3/spectrum.02186-23.f004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ec45/10715165/dbacc715cd61/spectrum.02186-23.f005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ec45/10715165/5113584c5fee/spectrum.02186-23.f006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ec45/10715165/859a5fcad89b/spectrum.02186-23.f007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ec45/10715165/cbca90b38a37/spectrum.02186-23.f008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ec45/10715165/ab71205d95f4/spectrum.02186-23.f001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ec45/10715165/198c320d7a79/spectrum.02186-23.f002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ec45/10715165/f94fd59b1e0d/spectrum.02186-23.f003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ec45/10715165/41ad3bb904f3/spectrum.02186-23.f004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ec45/10715165/dbacc715cd61/spectrum.02186-23.f005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ec45/10715165/5113584c5fee/spectrum.02186-23.f006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ec45/10715165/859a5fcad89b/spectrum.02186-23.f007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ec45/10715165/cbca90b38a37/spectrum.02186-23.f008.jpg

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