Center for iPS Cell Research and Application, Kyoto University, 53 Kawahara-cho, Shogoin, Sakyo-ku, Kyoto 606-8507, Japan.
Center for iPS Cell Research and Application, Kyoto University, 53 Kawahara-cho, Shogoin, Sakyo-ku, Kyoto 606-8507, Japan.
Curr Opin Genet Dev. 2023 Dec;83:102126. doi: 10.1016/j.gde.2023.102126. Epub 2023 Oct 7.
In 2021, we showed that naive human pluripotent stem cells (PSCs) can differentiate into trophoblasts via trophectoderm (TE)-like cells. Since TE is a pre-implantation stage of trophoblasts constituting blastocysts, naive human PSCs are an invaluable tool for understanding the entire process of trophoblast development. It has been reported for many years that primed human PSCs can also differentiate into the trophoblast lineage. The in vitro differentiation of naive and primed human PSCs hints at the possibility that human pre- and even post-implantation epiblasts retain the differentiation potential into the trophoblast lineages in vivo. Here, we review the in vitro specification of trophoblasts from human PSCs. Moreover, we discuss the different trophoblast differentiation pathways from naive and primed PSCs.
2021 年，我们证明了原始人类多能干细胞（PSCs）可以通过滋养外胚层（TE）样细胞分化为滋养层细胞。由于 TE 是构成囊胚的滋养层的着床前阶段，原始人类 PSCs 是理解滋养层发育全过程的宝贵工具。多年来已有报道称，已初始化的人类 PSCs 也可以分化为滋养层谱系。原始和初始化人类 PSCs 的体外分化暗示了人类甚至着床后内细胞团在体内保留向滋养层谱系分化的潜力。在这里，我们回顾了人类 PSCs 向滋养层细胞的体外特化。此外，我们还讨论了原始和初始化 PSCs 之间不同的滋养层分化途径。
