Xu B L, Ling S Q, Zhang Y, Liu X C, Luo Y, Yao X
Hospital for Skin Diseases, Institute of Dermatology, Chinese Academy of Medical Sciences and Peking Union Medical College, Nanjing 210042, China.
Dermatology Hospital, Southern Medical University, Guangzhou 510091, China.
Zhonghua Yi Xue Za Zhi. 2023 Oct 17;103(38):3041-3046. doi: 10.3760/cma.j.cn112137-20230724-00084.
To explore the role of Langerin in mediating epicutaneous sensitization of atopic dermatitis (AD) in mouse model. Mice were topically treated with calcipotriol (MC903) plus ovalbumin (OVA) on the ears to establish AD mouse models, and mice were divided into wild-type control group, wild-type AD group, Langerin knockout control group, and Langerin knockout AD group. Changes of lesion were daily observed. Infiltration of inflammatory cells, mRNA expression of Tslp, Il4, Il13, Il17a, and Il22, levels of serum total IgE, OVA-specific IgE (sIgE), OVA sIgG1 and OVA sIgG2a, proportion of regulatory T (Treg) cells in cervical draining lymph nodes were evaluated at the end of model preparation. Skin tumidness and thickness, dermal inflammatory cells infiltration, the mRNA expression levels of Tslp, Il4, Il13, Il17a and Il22 in wild-type AD groups were higher than those in wild-type control groups, with (1.80±0.66, 1.64±0.25, 1.71±0.54, 2.41±0.23, 2.49±0.32) and (0.53±0.45, 0.85±0.29, 0.73±0.50, 0.72±0.25, 0.56±0.29), respectively (all <0.05) In addition, the levels of serum total IgE, OVA sIgE and OVA sIgG1 in wild-type AD groups were higher than those in wild-type control groups, with [(1 216.00±572.70) ng/ml, (597.00±538.30) ng/ml, 1.59±0.09] and [(24.22±35.04) ng/ml, (20.01±41.71) ng/ml, 1.16±0.03], respectively (all <0.05). In Langerin knockout mice, compared to wild-type mice, skin erythema, skin tumidness, epidermal thickening, inflammatory cell infiltration were more obvious; the mRNA expression levels of Tslp, Il4, Il13, Il17a and Il22 were upregulated with (8.19±6.44, 2.53±0.69, 2.82±0.73, 3.94±1.32, 3.80±1.43) (all 0.05); the levels of serum total IgE, OVA sIgE and OVA sIgG1 were significantly increased with (2 508.00±657.10) ng/ml, (1 808.00±470.70) ng/ml, (1.73±0.09) (all 0.05); the number of CD4CD25CD127Treg cells were decreased significantly with (13.25±0.96)% and (15.31±1.47)%, respectively (0.05). Langerin is involved in mediating epicutaneous sensitization of the AD mouse model and plays a negative immunoregulatory role.
探讨朗格汉斯细胞凝集素（Langerin）在介导小鼠模型中特应性皮炎（AD）经皮致敏中的作用。将小鼠耳部局部用卡泊三醇（MC903）加卵清蛋白（OVA）处理以建立AD小鼠模型，小鼠分为野生型对照组、野生型AD组、Langerin基因敲除对照组和Langerin基因敲除AD组。每天观察皮损变化。在模型制备结束时，评估炎症细胞浸润、胸腺基质淋巴细胞生成素（Tslp）、白细胞介素4（Il4）、白细胞介素13（Il13）、白细胞介素17a（Il17a）和白细胞介素22（Il22）的mRNA表达、血清总IgE、OVA特异性IgE（sIgE）、OVA sIgG1和OVA sIgG2a水平、颈部引流淋巴结中调节性T（Treg）细胞的比例。野生型AD组的皮肤肿胀和厚度、真皮炎症细胞浸润、Tslp、Il4、Il13、Il17a和Il22的mRNA表达水平高于野生型对照组，分别为（1.80±0.66、1.64±0.25、1.71±0.54、2.41±0.23、2.49±0.32）和（0.53±0.45、0.85±0.29、0.73±0.50、0.72±0.25、0.56±0.29）（均P<0.05）。此外，野生型AD组的血清总IgE、OVA sIgE和OVA sIgG1水平高于野生型对照组，分别为[（1216.00±572.70）ng/ml、（597.00±538.30）ng/ml、1.59±0.09]和[（24.22±35.04）ng/ml、（20.XX±41.71）ng/ml、1.16±0.03]（均P<0.05）。在Langerin基因敲除小鼠中，与野生型小鼠相比，皮肤红斑、皮肤肿胀、表皮增厚、炎症细胞浸润更明显；Tslp、Il4、Il13、Il17a和Il22的mRNA表达水平上调，分别为（8.19±6.44、2.53±0.69、2.82±0.73、3.94±1.32、3.80±1.43）（均P<0.05）；血清总IgE、OVA sIgE和OVA sIgG1水平显著升高，分别为（2508.00±657.10）ng/ml、（1808.00±470.70）ng/ml、（1.73±0.09）（均P<0.05）；CD4CD25CD127Treg细胞数量显著减少，分别为（13.25±0.96）%和（1SX±1.47）%（P<0.05）。Langerin参与介导AD小鼠模型的经皮致敏并发挥负性免疫调节作用。
