Young R A, Mehendale H M
Food Chem Toxicol. 1986 Aug;24(8):863-8. doi: 10.1016/0278-6915(86)90078-5.
The effects of the cyclodiene pesticide, endrin, and its aldehyde and ketone metabolites on hepatobiliary function and CCl4-induced hepatotoxicity were investigated in Sprague-Dawley rats. The rats were given control diet or diets containing 5 or 10 ppm endrin, 10 ppm endrin aldehyde or 5 ppm endrin ketone for 15 days. Three to six rats from each treatment group were given a single ip dose (100 microliter/kg body weight) of CCl4 in corn oil (1 ml/kg) on day 15. Levels of serum glutamic-oxalacetic transaminase (SGOT), glutamic-pyruvic transaminase (SGPT), isocitrate dehydrogenase and ornithine-carbamyl transferase, bile flow and biliary excretion of an anionic model compound, phenolphthalein glucuronide (PG), were measured on day 16. Dietary treatment with endrin at either dose level did not significantly elevate serum enzyme levels, while endrin aldehyde produced a slight increase in SGOT and SGPT and endrin ketone produced a small elevation in SGPT levels. Treatment with endrin aldehyde or endrin ketone did not result in significant alterations of bile flow or biliary PG excretion. Treatment with 5 ppm endrin produced a significant reduction in bile flow and a corresponding reduction in PG excretion by male rats, whereas treatment with 10 ppm endrin reduced only the PG excretion by male rats. Female rats treated with 5 or 10 ppm endrin showed a dose-dependent choleretic effect with a commensurate increase in PG excretion. With the exception of a further slight reduction in PG excretion by male rats, treatment with the endrin or endrin derivative did not potentiate CCl4-induced alterations in hepatobiliary functions. Although the levels of some serum enzymes of rats given endrin or endrin derivatives plus CCl4 were elevated over those of rats given CCl4 alone, the increases were not of the magnitude of those that have been reported previously for chlordecone. Generally, female rats challenged with CCl4 or endrin/CCl4 exhibited greater increases in serum enzyme levels than did male rats given corresponding treatments.
在斯普拉格 - 道利大鼠中研究了环二烯农药异狄氏剂及其醛和酮代谢产物对肝胆功能以及四氯化碳诱导的肝毒性的影响。给大鼠喂食对照饮食或含有5或10 ppm异狄氏剂、10 ppm异狄氏剂醛或5 ppm异狄氏剂酮的饮食，持续15天。在第15天，给每个处理组的3至6只大鼠腹腔注射一次（100微升/千克体重）玉米油（1毫升/千克）中的四氯化碳。在第16天测量血清谷草转氨酶（SGOT）、谷丙转氨酶（SGPT）、异柠檬酸脱氢酶和鸟氨酸氨甲酰转移酶水平、胆汁流量以及阴离子模型化合物酚酞葡糖苷酸（PG）的胆汁排泄。用任一剂量水平的异狄氏剂进行饮食处理均未显著提高血清酶水平，而异狄氏剂醛使SGOT和SGPT略有升高，异狄氏剂酮使SGPT水平有小幅升高。用异狄氏剂醛或异狄氏剂酮处理未导致胆汁流量或胆汁PG排泄的显著改变。用5 ppm异狄氏剂处理使雄性大鼠的胆汁流量显著减少，PG排泄相应减少，而用10 ppm异狄氏剂处理仅使雄性大鼠的PG排泄减少。用5或10 ppm异狄氏剂处理的雌性大鼠表现出剂量依赖性的利胆作用，PG排泄相应增加。除雄性大鼠的PG排泄进一步略有减少外，用异狄氏剂或异狄氏剂衍生物处理未增强四氯化碳诱导的肝胆功能改变。尽管给予异狄氏剂或异狄氏剂衍生物加四氯化碳的大鼠的一些血清酶水平高于仅给予四氯化碳的大鼠，但升高幅度不及先前报道的十氯酮。一般来说，用四氯化碳或异狄氏剂/四氯化碳攻击的雌性大鼠血清酶水平的升高幅度大于接受相应处理的雄性大鼠。
