<institution content-type="university">College of Agriculture, Guangxi University</institution>, <city>Nanning</city> <postal-code>530004</postal-code>, <country>China</country>; and <institution content-type="university">Guangxi Botanical Garden of Medicinal Plants</institution>, <city>Nanning</city> <postal-code>530023</postal-code>, <country>China</country>.
<institution content-type="university">College of Agriculture, Guangxi University</institution>, <city>Nanning</city> <postal-code>530004</postal-code>, <country>China</country>; and <institution content-type="university">Guangdong Key Laboratory of High Technology for Plant Protection, Institute of Plant Protection, Guangdong Academy of Agricultural Sciences</institution>, <city>Guangzhou</city> <postal-code>510640</postal-code>, <country>China</country>.
Funct Plant Biol. 2023 Dec;50(12):1047-1061. doi: 10.1071/FP23084.
Anthracnose caused by Colletotrichum gloeosporioides critically threatens the growth and commercial cultivation of Sarcandra glabra . However, the defence responses and underlying mechanisms remain unclear. Herein, we aimed to investigate the molecular reprogramming in S. glabra leaves infected with C. gloeosporioides . Leaf tissues at 0, 24 and 48h post-inoculation (hpi) were analysed by combining RNA sequencing and Tandem Mass Tag-based liquid chromatography with tandem mass spectrometry. In total, 18 441 and 25 691 differentially expressed genes were identified at 24 and 48hpi compared to 0hpi (uninoculated control), respectively. In addition, 1240 and 1570 differentially abundant proteins were discovered at 24 and 48hpi compared to 0hpi, respectively. Correlation analysis revealed that transcription and translation levels were highly consistent regarding repeatability and expression. Analyses using databases KEGG and iPATH revealed tricitric acid cycle, glycolysis/gluconeogenesis and phenylpropanoid biosynthesis were induced, whereas photosynthesis and tryptophan were suppressed. Enzymatic activity assay results were consistent with the upregulation of defence-related enzymes including superoxide dismutases, catalases, peroxidases and chitinases. The transcriptome expression results were additionally validated by quantitative real-time polymerase chain reaction analyses. This study provides insights into the molecular reprogramming in S. glabra leaves during infection, which lay a foundation for investigating the mechanisms of host-Colletotrichum interactions and breeding disease-resistant plants.
炭疽病由胶孢炭疽菌引起，严重威胁着肿节风的生长和商业种植。然而，其防御反应和潜在机制尚不清楚。在此，我们旨在研究胶孢炭疽菌感染肿节风叶片后的分子重编程。通过结合 RNA 测序和串联质量标签（TMT）基于液相色谱与串联质谱分析，对接种后 0、24 和 48 小时（hpi）的叶片组织进行分析。与未接种（对照）的 0hpi 相比，分别在 24 和 48 hpi 时鉴定出 18441 和 25691 个差异表达基因，在 24 和 48 hpi 时分别鉴定出 1240 和 1570 个差异丰度蛋白。相关性分析表明转录和翻译水平在重复性和表达方面高度一致。KEGG 和 iPATH 数据库分析表明，三羧酸循环、糖酵解/糖异生和苯丙素生物合成被诱导，而光合作用和色氨酸被抑制。酶活性测定结果与防御相关酶（包括超氧化物歧化酶、过氧化氢酶、过氧化物酶和几丁质酶）的上调一致。通过定量实时聚合酶链反应分析进一步验证了转录组表达结果。本研究为研究宿主-胶孢炭疽菌相互作用的机制和培育抗病植物提供了基础，深入了解了肿节风叶片在感染过程中的分子重编程。
