通过高效薄层色谱法对海洛因和可卡因样品进行定量光密度测定。

A quantitative densitometric determination of heroin and cocaine samples by high-performance thin-layer chromatography.

作者信息

Della Casa E, Martone G

出版信息

Forensic Sci Int. 1986 Oct;32(2):117-20. doi: 10.1016/0379-0738(86)90147-7.

Abstract

The traditional thin-layer chromatography (TLC) is a sensitive technique frequently used in chemical analysis for semiquantitative determinations. In the last years a high-performance thin-layer chromatography system (HPTLC) has been developed. It assures quantitative determinations with direct densitometric measurements of chromatographic spots in situ on the plate. Here, we wish to report a method for the drug determination in street samples, that employs a CAMAG TLC/HPTLC Scanner Photodensitometer and a CAMAG LINOMAT III for sample application. Samples of illicit drug traffic (50 samples of street heroin and 30 samples of street cocaine) are tested and the gas-liquid chromatography (GLC) and HPTLC determinations are compared. The HPTLC method has proven to be sensitive, precise and accurate for quantitative determinations of drugs in street samples. Furthermore this method may be useful in forensic chemistry to show a "fingerprint" of each sample.

摘要

传统的薄层色谱法（TLC）是化学分析中常用于半定量测定的一种灵敏技术。近年来，已开发出一种高效薄层色谱系统（HPTLC）。它通过对板上原位色谱斑点进行直接光密度测量来确保定量测定。在此，我们希望报告一种用于测定街头样品中药物的方法，该方法采用CAMAG TLC/HPTLC扫描仪光密度计和CAMAG LINOMAT III进行样品点样。对非法毒品交易的样品（50份街头海洛因样品和30份街头可卡因样品）进行了检测，并比较了气液色谱法（GLC）和HPTLC测定结果。事实证明，HPTLC方法对于定量测定街头样品中的药物灵敏、精确且准确。此外，该方法在法医化学中可能有助于显示每个样品的“指纹”。