Kumar Pankaj, Sharma Shivali, Tripathi Vibhay Nath
Department of Botany, Guru Ghasidas University, Bilaspur, India 495009.
Bioinformation. 2023 Feb 28;19(2):199-205. doi: 10.6026/97320630019199. eCollection 2023.
Quorum sensing is a phenomena used by bacteria for regulation of some important characteristics coordinately. Gram-negative and Gram-positive bacteria, both utilize this system of communication, but they use different signal molecules for this, i.e. Gram-negative bacteria preferentially use acyl homoserine lactone (AHL). Rhizospheric soil is rich in bacterial diversity, with the presence of both types of bacteria. It will be useful if we know the quorum sensing based communications among bacteria present in rhizospheric soil. This work was undertaken with the idea to explore the diversity of AHL producing bacteria in soil. For this, bacteria were isolated from rice rhizosphere from field located in Dhamtari district of Chhattisgarh. To study quorum sensing, detection of AHL production was performed by colorimetry method. Out of total 29 bacterial isolates, only three Gram-negative bacteria showed positive result for AHLs production. These 3 isolates were selected for further study. The isolates were characterized by biochemical test. Other characteristics of these bacteria were also tested, like antibiotic resistance profile, indole test, and capability to biofilm formation, phosphate solubilization as well as their growth in different specific culture media. The Results suggest that AHLs producing bacteria may belong to family of sp., which are reported in different researches to be part of the PGPR group of organisms. This work forms the base to study the cell-cell communication in plant rhizospheres.
群体感应是细菌用于协调调节某些重要特性的一种现象。革兰氏阴性菌和革兰氏阳性菌都利用这种通讯系统，但它们为此使用不同的信号分子，即革兰氏阴性菌优先使用酰基高丝氨酸内酯（AHL）。根际土壤中细菌多样性丰富，两种类型的细菌都存在。如果我们了解根际土壤中细菌之间基于群体感应的通讯，将会很有帮助。这项工作旨在探索土壤中产生AHL的细菌的多样性。为此，从恰蒂斯加尔邦达姆塔里区田间的水稻根际分离细菌。为了研究群体感应，采用比色法检测AHL的产生。在总共29株细菌分离物中，只有三株革兰氏阴性菌对AHL的产生呈阳性结果。选择这3株分离物进行进一步研究。通过生化试验对分离物进行表征。还测试了这些细菌的其他特性，如抗生素抗性谱、吲哚试验、生物膜形成能力、磷溶解能力以及它们在不同特定培养基中的生长情况。结果表明，产生AHL的细菌可能属于sp.科，在不同研究中报道它们是植物根际促生菌（PGPR）生物群体的一部分。这项工作为研究植物根际中的细胞间通讯奠定了基础。
