School of Life Sciences, Lanzhou University, Lanzhou, China.
Institute of Modern Physics, Chinese Academy of Sciences, Lanzhou, China.
Photochem Photobiol. 2024 May-Jun;100(3):604-621. doi: 10.1111/php.13863. Epub 2023 Oct 10.
Although blue light can damage the skin to a certain extent, the pathogenesis of its damage remains still unclear. The available evidence suggests that oxidative stress may be the main cause of its damage. Lycium barbarum polysaccharide (LBP) has antioxidative effects in a variety of cells. In this paper, we investigated the protective role of LBP and its mechanism of action related to mitophagy in blue-light-damaged skin cells. The findings indicated that in HaCaT cells and mouse skin, LBP pretreatment was effective in reducing blue-light-induced apoptosis and ameliorating the elevated level of cellular autophagy/mitophagy caused by excessive blue light exposure. The markers reactive oxygen species (ROS), superoxide dismutase (SOD), and malondialdehyde (MDA) were used to assess oxidative stress. LBP could effectively inhibit blue-light-induced oxidative stress. It was also found that blue light exposure caused mitochondrial dysfunction in HaCaT cells, including increased intracellular calcium ion levels and decreased mitochondrial membrane potential. LBP pretreatment significantly relieved mitochondrial dysfunction in HaCaT cells. These findings imply that LBP pretreatment protects skin cells from damage induced by blue light irradiation and that mitophagy may be a significant factor in skin photodamage.
尽管蓝光在一定程度上会损伤皮肤，但损伤的发病机制尚不清楚。现有证据表明，氧化应激可能是其损伤的主要原因。枸杞多糖（LBP）在多种细胞中具有抗氧化作用。本文研究了 LBP 在蓝光损伤皮肤细胞中的保护作用及其与自噬相关的作用机制。研究结果表明，在 HaCaT 细胞和小鼠皮肤中，LBP 预处理可有效减少蓝光诱导的细胞凋亡，并改善因过度暴露于蓝光而引起的细胞自噬/线粒体自噬水平升高。采用活性氧（ROS）、超氧化物歧化酶（SOD）和丙二醛（MDA）标志物评估氧化应激。LBP 可有效抑制蓝光诱导的氧化应激。还发现蓝光照射导致 HaCaT 细胞线粒体功能障碍，包括细胞内钙离子水平升高和线粒体膜电位降低。LBP 预处理可显著缓解 HaCaT 细胞的线粒体功能障碍。这些发现表明，LBP 预处理可保护皮肤细胞免受蓝光照射引起的损伤，而线粒体自噬可能是皮肤光损伤的重要因素。
