Graduate School of Pharmaceutical Sciences, Tohoku University, Sendai, Japan.
Department of Biology, The University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA.
Genes Cells. 2023 Dec;28(12):857-867. doi: 10.1111/gtc.13072. Epub 2023 Oct 10.
Drosophila imaginal disc cells can change their identity under stress conditions through transdetermination (TD). Research on TD can help elucidate the in vivo process of cell fate conversion. We previously showed that the overexpression of winged eye (wge) induces eye-to-wing TD in the eye disc and that an insulin-like peptide, Dilp8, is then highly expressed in the disc. Although Dilp8 is known to mediate systemic developmental delay via the Lgr3 receptor, its role in TD remains unknown. This study showed that Dilp8 is expressed in specific cells that do not express eye or wing fate markers during Wge-mediated TD and that the loss of Dilp8 impairs the process of eye-to-wing transition. Thus, Dilp8 plays a pivotal role in the cell fate conversion under wge overexpression. Furthermore, we found that instead of Lgr3, another candidate receptor, Drl, is involved in Wge-mediated TD and acts locally in the eye disc cells. We propose a model in which Dilp8-Drl signaling organizes cell fate conversion in the imaginal disc during TD.
果蝇的 imaginal disc 细胞在应激条件下可以通过转决定（TD）改变其身份。对 TD 的研究有助于阐明细胞命运转化的体内过程。我们之前曾表明，winged eye（wge）的过表达会诱导眼盘的眼-翅 TD，并且 disc 中高度表达胰岛素样肽 Dilp8。虽然已知 Dilp8 通过 Lgr3 受体介导全身发育延迟，但它在 TD 中的作用仍不清楚。本研究表明，在 Wge 介导的 TD 过程中，Dilp8 表达在不表达眼或翅命运标记的特定细胞中，并且 Dilp8 的缺失会损害眼-翅转变的过程。因此，Dilp8 在 wge 过表达下的细胞命运转换中起着关键作用。此外，我们发现，候选受体 Drl 而不是 Lgr3，参与了 Wge 介导的 TD，并在眼盘细胞中局部发挥作用。我们提出了一个模型，其中 Dilp8-Drl 信号在 TD 期间组织 imaginal disc 中的细胞命运转换。
