Department of Neurology, The Eighth Affiliated Hospital, Sun Yat-sen University, Shenzhen, Guangdong, China.
Department of Neurology, Huizhou Central People's Hospital, Huizhou City, Guangdong, China.
Folia Neuropathol. 2023;61(3):242-248. doi: 10.5114/fn.2023.130264.
Involving in the immune response after cerebral infarction, astrocytes could secrete large amounts of pro- and anti-inflammatory factors. The aim of this study is to investigate the effect of Wnt3a intervention on the inflammatory response of oxygen-glucose deprivation (OGD) followed by reoxygenation (OGD/R) astrocyte model, and to provide a new target for immunoprotective treatment of cerebral infarction. We constructed the OGD/R rat astrocyte model, the astrocytes were treated by different concentrations of glucose (25, 50, 100 mM) intervened with/without Wnt3a (25 µg/ml). Microscope was used to observe the cell survival in rat astrocytes. The relative expression of inflammatory factors (TNF-a, IL-6, HIF-a) in rat astrocytes was detected by qRT-PCR. The expression of inflammatory factors such as TNF-a, IL-6 and HIF-a in rat astrocytes was increased after OGD/R treatment. The Wnt3a intervention promoted cell survival and decreased the expression of inflammatory factors in rat astrocytes induced by OGD/R. There is a neuroprotective effect that Wnt3a intervention could reduce inflammatory response in the OGD/R rat astrocyte model.
星形胶质细胞参与脑梗死后的免疫反应，可大量分泌促炎和抗炎因子。本研究旨在探讨 Wnt3a 干预对氧葡萄糖剥夺（OGD）后再复氧（OGD/R）星形胶质细胞模型炎症反应的影响，为脑梗死的免疫保护治疗提供新靶点。构建 OGD/R 大鼠星形胶质细胞模型，用不同浓度葡萄糖（25、50、100 mM）干预并用/不用 Wnt3a（25 µg/ml）处理。用显微镜观察大鼠星形胶质细胞的细胞存活率。用 qRT-PCR 检测大鼠星形胶质细胞中炎症因子（TNF-a、IL-6、HIF-a）的相对表达。OGD/R 处理后，大鼠星形胶质细胞中炎症因子 TNF-a、IL-6 和 HIF-a 的表达增加。Wnt3a 干预促进细胞存活，降低 OGD/R 诱导的大鼠星形胶质细胞中炎症因子的表达。Wnt3a 干预在 OGD/R 大鼠星形胶质细胞模型中具有减轻炎症反应的神经保护作用。
