Department of Biotechnology, Science Campus, Alagappa University, Karaikudi, Tamil Nadu, India.
National Institute of Pharmaceutical Education and Research, Kolkata, West Bengal, India.
Mol Omics. 2024 Jan 15;20(1):48-63. doi: 10.1039/d3mo00167a.
The neural pathways of play a crucial role in regulating host immunity and inflammation during pathogenic infections. To understand the major neuro-immune signaling pathways, this study aimed to identify the key regulatory proteins in the host during infection. We used high-throughput label-free quantitative proteomics and identified 69 differentially expressed proteins. KEGG analysis revealed that elicited host immune signaling cascades primarily including mTOR signaling, axon regeneration, metabolic pathways ( and ), calcium signaling , and longevity regulating pathways (), respectively. The abrogation in functional loss of mTOR-associated players deciphered that infection negatively regulated the lifespan of mutant worms (, and ), including physiological aberrations, such as reduced pharyngeal pumping and egg production. Additionally, the candidate pathway proteins were validated by transcriptional profiling of their corresponding genes. Furthermore, immunoblotting showed the downregulation of mTORC2/SGK-1 during the later hours of pathogen exposure. Overall, our findings profoundly provide an understanding of the specificity of proteome imbalance in affecting neuro-immune regulations during infection.
玩耍的神经通路在调节宿主免疫和炎症方面起着至关重要的作用，尤其是在病原感染期间。为了了解主要的神经免疫信号通路，本研究旨在确定宿主在感染期间的关键调节蛋白。我们使用高通量无标记定量蛋白质组学技术，鉴定出 69 种差异表达蛋白。KEGG 分析显示，感染引发了宿主免疫信号级联反应，主要包括 mTOR 信号通路、轴突再生、代谢途径（和）、钙信号通路和长寿调节途径（）。mTOR 相关蛋白功能丧失的阻断表明，感染会负调控突变体蠕虫的寿命（、和），包括生理异常，如咽部抽吸和产卵减少。此外，通过相应基因的转录谱对候选通路蛋白进行了验证。此外，免疫印迹显示在病原体暴露后的后期，mTORC2/SGK-1 下调。总的来说，我们的研究结果深入了解了在感染过程中，蛋白质组失衡对神经免疫调节的特异性影响。
