Department of Microbiology and Biotechnology, University Hamburg , Hamburg, Germany.
Cellular Microbiology, Priority Research Area Infections, Research Center Borstel, Leibniz Lung Center,Leibniz Research Alliance Infection , Borstel Gemany , Borstel, Germany.
Microbiol Spectr. 2023 Dec 12;11(6):e0085923. doi: 10.1128/spectrum.00859-23. Epub 2023 Oct 11.
In the past, studies have focused on bacterial pathogenicity in mono-species infections, in part ignoring the clinical relevance of diseases caused by more than one pathogen (i.e., polymicrobial infections). However, it is now common knowledge that multiple bacteria species are often involved in the course of an infection. For treatment of such infections, it is absolutely important to understand the dynamics of species interactions at possible infection sites and the molecular mechanisms behind these interactions. Here, we studied the impact of on its commensals and in multispecies biofilms. We analyzed the 3D structural architectures of dual- and triple-species biofilms, niche formation within the biofilms, and the interspecies interactions on a molecular level. RNAseq data identified key genes involved in multispecies biofilm formation and interaction as potential drug targets for the clinical combat of multispecies infection with these major pathogens.
过去，研究主要集中在单一种群感染中的细菌致病性，部分忽略了由一种以上病原体引起的疾病的临床相关性（即，多微生物感染）。然而，现在人们普遍认识到，多种细菌物种通常参与感染过程。对于此类感染的治疗，绝对有必要了解可能感染部位的物种相互作用的动态以及这些相互作用背后的分子机制。在这里，我们研究了 对其共生菌 和 的影响。我们分析了双种和三种生物膜的 3D 结构架构，生物膜内的小生境形成以及种间相互作用的分子水平。RNAseq 数据鉴定了多物种生物膜形成和相互作用中涉及的关键基因，这些基因可能成为针对这些主要病原体的多物种感染的临床治疗的潜在药物靶点。
