Department of Biotechnology & Microbiology, Karnatak University, Dharwad, India;
KLE College of Pharmacy, Hubballi (A constituent Unit of KLE Academy of Higher Education and Research), Belagavi, India.
J Biomol Struct Dyn. 2024;42(22):12271-12281. doi: 10.1080/07391102.2023.2268218. Epub 2023 Oct 11.
This research is carried out to explore the hypoglycemic activity of seed extracts by , , and methods. For studies the α-amylase and α-glucosidase enzyme inhibition assays were employed. For studies 30 alloxan induced Wistar rats were used. They were orally administered with glibenclamide and low/high dose of the extracts and were monitored regularly for the change in blood glucose levels for about 28 days. The molecular docking was conducted to evaluate the binding interaction of 1,2,3-Benzenetriol with human pancreatic α-amylase and α-glucosidase. It was found that all the extracts were able to inhibit the α-amylase and α-glucosidase enzymes. Among which the acetone extract showed greater inhibition with 72.52 ± 0.51% and 63.02 ± 0.73% for both the enzymes, respectively. There was significant ( < 0.05) reduction in blood glucose levels in the rats administered with glibenclamide and extracts. docking results revealed that the compound 1,2,3-Benzenetriol exhibited the highest binding affinity for human pancreatic α-amylase with binding energy -7.7 kcal/mol. Thus suggesting the utilization of seeds in the management of diabetes mellitus.Communicated by Ramaswamy H. Sarma.
本研究旨在通过 、 、 方法探索种子提取物的降血糖活性。对于 研究，采用了α-淀粉酶和α-葡萄糖苷酶抑制试验。对于 研究，使用了 30 只链脲佐菌素诱导的 Wistar 大鼠。它们口服给予格列本脲和低/高剂量的提取物，并定期监测大约 28 天内血糖水平的变化。进行了分子对接，以评估 1,2,3-苯三醇与人胰腺α-淀粉酶和α-葡萄糖苷酶的结合相互作用。结果发现，所有提取物都能够抑制α-淀粉酶和α-葡萄糖苷酶。其中，丙酮提取物对两种酶的抑制率分别为 72.52±0.51%和 63.02±0.73%。给予格列本脲和提取物的大鼠血糖水平显著降低（<0.05）。对接结果表明，化合物 1,2,3-苯三醇与人胰腺α-淀粉酶具有最高的结合亲和力，结合能为-7.7kcal/mol。因此，建议在糖尿病的治疗中使用 种子。由 Ramaswamy H. Sarma 传达。
