来自海胆卵的45K分子量肌动蛋白调节蛋白在钙离子存在的情况下与肌动蛋白形成复合物。
The 45K molecular weight actin-modulating protein from sea urchin eggs forms a complex with actin in the presence of calcium ions.
作者信息
Ohnuma M, Mabuchi I
出版信息
J Biochem. 1986 Sep;100(3):817-20. doi: 10.1093/oxfordjournals.jbchem.a121776.
The 45K protein from sea urchin eggs forms a complex with actin in the presence of Ca2+. The fraction was not readily dissociated on depletion of Ca2+ by gel filtration, but was dissociated on dialysis against an EGTA-containing solution. The free 45K protein and the 45K protein-actin complex affect F-actin in different manners in the presence of Ca2+. The former severs F-actin in the presence of Ca2+ but not in its absence, while the latter caps the barbed end of F-actin in a Ca2+-independent manner thereby lowering the viscosity of F-actin slowly.
海胆卵中的45K蛋白在Ca2+存在的情况下与肌动蛋白形成复合物。通过凝胶过滤耗尽Ca2+时，该组分不易解离，但用含EGTA的溶液透析时会解离。在Ca2+存在的情况下，游离的45K蛋白和45K蛋白 - 肌动蛋白复合物以不同方式影响F - 肌动蛋白。前者在Ca2+存在时切断F - 肌动蛋白，而在其不存在时则不然，而后者以不依赖Ca2+的方式封闭F - 肌动蛋白的刺端，从而缓慢降低F - 肌动蛋白的粘度。
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