State Key Laboratory of Respiratory Disease, National Clinical Research Center for Respiratory Disease, Guangzhou Institute of Respiratory Health, the First Affiliated Hospital of Guangzhou Medical University, Guangzhou Medical University, Guangzhou, China; Guangzhou Laboratory, No. 9 XingDaoHuanBei Road, Guangzhou International Bio Island, Guangzhou 510005, Guangdong Province, China.
State Key Laboratory of Respiratory Disease, National Clinical Research Center for Respiratory Disease, Guangzhou Institute of Respiratory Health, the First Affiliated Hospital of Guangzhou Medical University, Guangzhou Medical University, Guangzhou, China.
Vaccine. 2023 Nov 2;41(46):6863-6869. doi: 10.1016/j.vaccine.2023.09.059. Epub 2023 Oct 9.
New SARS-CoV-2 variants continue to prevail worldwide, and effective vaccines are needed to prevent an epidemic. mRNA vaccines are gradually being applied to the prevention and control of infectious diseases with significant safety and effectiveness. The spike (S) protein of SARS-CoV-2 is the main target of mRNA vaccine design, but the impact of the signal peptide (SP), transmembrane region (TM), and cytoplasmic tail (CT) on mRNA vaccine remains unclear. In this study, we constructed three forms of mRNA vaccines related to the S protein: full-length, deletion of the TM and CT, and simultaneous deletion of the SP, TM and CT, and compared their immunogenicity. Our experimental data show that full-length S protein and deletion of the TM and CT could effectively induce neutralizing antibody production in mice, while S protein without the SP and TM could not. This indicates that the S protein SP is necessary for the design of mRNA vaccine.
新型 SARS-CoV-2 变体继续在全球流行，需要有效的疫苗来预防疫情。mRNA 疫苗在传染病的预防和控制方面逐渐得到应用，具有显著的安全性和有效性。SARS-CoV-2 的刺突（S）蛋白是 mRNA 疫苗设计的主要目标，但信号肽（SP）、跨膜区（TM）和细胞质尾（CT）对 mRNA 疫苗的影响尚不清楚。在这项研究中，我们构建了三种与 S 蛋白相关的 mRNA 疫苗：全长、TM 和 CT 缺失，以及同时缺失 SP、TM 和 CT，并比较了它们的免疫原性。我们的实验数据表明，全长 S 蛋白和 TM 和 CT 缺失可以有效诱导小鼠产生中和抗体，而没有 SP 和 TM 的 S 蛋白则不能。这表明 S 蛋白的 SP 对于 mRNA 疫苗的设计是必要的。
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