分泌型钙结合磷蛋白（scpp）基因在牙鲆形成和替换咽齿过程中的表达。

Expression of secretory calcium-binding phosphoprotein (scpp) genes in medaka during the formation and replacement of pharyngeal teeth.

机构信息

Department of Oral and Maxillofacial Anatomy, Institute of Biomedical Sciences, Tokushima University Graduate School, 3-18-15, Kuramoto-cho, Tokushima-shi, Tokushima, 770-8504, Japan.

Oral Surgery Department, St. Luke's International Hospital, 9-1, Akashi-cho, Chuo-ku, Tokyo, 104-8560, Japan.

出版信息

BMC Oral Health. 2023 Oct 11;23(1):744. doi: 10.1186/s12903-023-03498-7.

DOI:10.1186/s12903-023-03498-7

PMID:37821862

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10568847/

Abstract

BACKGROUND

Analyses of tooth families and tooth-forming units in medaka with regard to tooth replacement cycles and the localization of odontogenic stem cell niches in the pharyngeal dentition clearly indicate that continuous tooth replacement is maintained. The secretory calcium-binding phosphoprotein (scpp) gene cluster is involved in the formation of mineralized tissues, such as dental and bone tissues, and the genes encoding multiple SCPPs are conserved in fish, amphibians, reptiles, and mammals. In the present study, we examined the expression patterns of several scpp genes in the pharyngeal teeth of medaka to elucidate their roles during tooth formation and replacement.

METHODS

Himedaka (Japanese medaka, Oryzias latipes) of both sexes (body length: 28 to 33 mm) were used in this study. Real-time quantitative reverse transcription-polymerase chain reaction (PCR) (qPCR) data were evaluated using one-way analysis of variance for multi-group comparisons, and the significance of differences was determined by Tukey's comparison test. The expression of scpp genes was examined using in situ hybridization (ISH) with a digoxigenin-labeled, single-stranded antisense probe.

RESULTS

qPCR results showed that several scpp genes were strongly expressed in pharyngeal tissues. ISH analysis revealed specific expression of scpp1, scpp5, and sparc in tooth germ, and scpp5 was continually expressed in the odontoblasts of teeth attached to pedicles, but not in the osteoblasts of pedicles. In addition, many scpp genes were expressed in inner dental epithelium (ide), but not in odontoblasts, and scpp2 consistently showed epithelial-specific expression in the functional teeth. Taken together, these data indicate that specific expression of scpp2 and scpp5 may play a critical role in pharyngeal tooth formation in medaka.

CONCLUSION

We characterized changes in the expression patterns of scpp genes in medaka during the formation and replacement of pharyngeal teeth.

摘要

背景

对牙列和牙形成单位的分析表明，牙鲆的牙齿替换周期和咽齿中牙源性干细胞龛的定位明确表明，连续的牙齿替换得以维持。分泌型钙结合磷蛋白 (scpp) 基因簇参与矿化组织（如牙齿和骨骼组织）的形成，鱼类、两栖动物、爬行动物和哺乳动物中编码多个 scpp 的基因是保守的。在本研究中，我们研究了 scpp 基因在牙鲆咽齿中的表达模式，以阐明它们在牙齿形成和替换过程中的作用。

方法

本研究使用了雌雄两性（体长：28 至 33 毫米）的 Himedaka（日本牙鲆，Oryzias latipes）。使用单向方差分析对实时定量逆转录聚合酶链反应 (qPCR) 数据进行评估，并通过 Tukey 比较检验确定差异的显著性。使用 DIG 标记的单链反义探针进行原位杂交 (ISH) 来检查 scpp 基因的表达。

结果

qPCR 结果表明，几个 scpp 基因在咽组织中强烈表达。ISH 分析显示 scpp1、scpp5 和 sparc 在牙原基中特异性表达，scpp5 在附着于蒂的牙齿的成牙本质细胞中持续表达，但在蒂的成骨细胞中不表达。此外，许多 scpp 基因在内釉上皮 (ide) 中表达，但不在成牙本质细胞中表达，而 scpp2 在功能性牙齿中始终表现出上皮特异性表达。总之，这些数据表明 scpp2 和 scpp5 的特异性表达可能在牙鲆咽齿形成中发挥关键作用。