Zhuo Guorong, Wang Lei, Ali Muhammad, Jing Zheng, Hassan Mohammad Farooque
College of Small Animal Science and Technology, Jiangsu Agri-Animal Husbandry Vocational College, Taizhou, China.
College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, China.
Front Vet Sci. 2023 Sep 26;10:1273944. doi: 10.3389/fvets.2023.1273944. eCollection 2023.
Hexavalent chromium Cr (VI) is one of the most hazardous heavy metals in the environment and is toxic to living organisms causing tissue damage, disruption of the intestinal microbiota and cancer. However, there is little information on the relationship between the Cr (VI) and broiler chickens. The current study was performed to investigate the effect of Cr (VI) on growth performance, serum biochemical analysis, histopathological observations, and metabolomics analysis in broilers. Results show that Cr (VI) exposure significantly decreased the body weight ( < 0.01) and caused liver damages in broilers. With the extension of Cr (VI) action time, the liver appeared obvious pathological changes, including hepatic cord disorder, incomplete hepatocyte additionally, decreased serum biochemical indices of calcium (Ca), phosphorus (P), total protein (TP), phosphatase (ALP), and globin (GLB) significantly ( < 0.01). Moreover, metabolomics analysis indicated that 29 differential metabolites were identified, such as phytosphingosine, L-Serine, 12, 13-DHOME, Alpha-dimorphecolic acid, L-Methionine, L-Phenylalanine, 3-Dehydroshikimate, L-Tyrosine, and N-Acetyl-L-phenylalanine were significantly decreased under the action of Cr (VI) ( < 0.05). These 29 differential metabolites are mainly involved in 35 metabolic pathways, such as aminoacyl-tRNA biosynthesis, phenylalanine metabolism, sphingolipid, and linoleic metabolism. The study revealed that exposure to Cr (VI) resulted in a decrease in growth performance and metabolism, with the hazards and toxicity in broiler chicken. The findings provided new insight and a comprehensive understanding of the relationship between Cr (VI) and broiler chickens.
六价铬（Cr(VI)）是环境中危害最大的重金属之一，对生物体有毒，会导致组织损伤、肠道微生物群紊乱和癌症。然而，关于Cr(VI)与肉鸡之间关系的信息很少。本研究旨在探讨Cr(VI)对肉鸡生长性能、血清生化分析、组织病理学观察和代谢组学分析的影响。结果表明，暴露于Cr(VI)会显著降低肉鸡体重（<0.01）并导致肝脏损伤。随着Cr(VI)作用时间的延长，肝脏出现明显的病理变化，包括肝索紊乱、肝细胞不完整，此外，血清中钙（Ca）、磷（P）、总蛋白（TP）、磷酸酶（ALP）和球蛋白（GLB）的生化指标显著下降（<0.01）。此外，代谢组学分析表明，共鉴定出29种差异代谢物，如植物鞘氨醇、L-丝氨酸、12,13-二羟基十八碳二烯酸、α-二异戊酸、L-蛋氨酸L-苯丙氨酸、3-脱氢莽草酸、L-酪氨酸和N-乙酰-L-苯丙氨酸在Cr(VI)作用下显著降低（<0.05）。这29种差异代谢物主要参与35条代谢途径,如氨酰-tRNA生物合成、苯丙氨酸代谢、鞘脂代谢和亚油酸代谢。该研究表明，暴露于Cr(VI)会导致肉鸡生长性能和代谢下降，并具有危害性和毒性。这些发现为理解Cr(VI)与肉鸡之间的关系提供了新的见解和全面的认识。
