Department of Laboratory Medicine, Nagano Children's Hospital , Azumino, Japan.
Life Science Research Center, Nagano Children's Hospital , Azumino, Japan.
Microbiol Spectr. 2023 Dec 12;11(6):e0216523. doi: 10.1128/spectrum.02165-23. Epub 2023 Oct 12.
We characterized serotype 24F sequence type (ST) 162 isolated from Japanese children with invasive pneumococcal disease (IPD). Owing to its highly invasive nature, serotype 24F is expected to be isolated from clinically significant cases. Serotype 24F ST162 isolates tested in the present study did not grow at 35°C in ambient air. Therefore, antimicrobial susceptibility testing using the broth microdilution method, which is usually conducted in ambient air, cannot be performed, posing a clinical challenge. Clinical practitioners and laboratory personnel should be aware of the epidemiological, bacteriological, and molecular characteristics of serotype 24F ST162. We believe that our findings can help diagnose and treat IPD caused by serotype 24F ST162, a serotype expected to become problematic in the post-13 valent pneumococcal conjugate vaccine era.
我们对从日本侵袭性肺炎球菌病（IPD）患儿中分离出的血清型 24F 序列型 162 进行了特征描述。由于其高度侵袭性，预计血清型 24F 将从临床上有意义的病例中分离出来。本研究中检测的血清型 24F ST162 分离株在环境空气中不能在 35°C 下生长。因此，通常在环境空气中进行的肉汤微量稀释法药敏试验无法进行，这对临床治疗带来了挑战。临床医生和实验室人员应了解血清型 24F ST162 的流行病学、细菌学和分子特征。我们相信我们的发现可以帮助诊断和治疗由血清型 24F ST162 引起的 IPD，这种血清型在 13 价肺炎球菌结合疫苗时代后预计会成为问题。
