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宽叶野豌豆萎蔫病毒 2 的混合 RNA 测序。

Hybrid RNA sequencing of broad bean wilt virus 2 from faba beans.

机构信息

NSW Department of Primary Industries, Biosecurity & Food Safety, Elizabeth Macarthur Agricultural Institute , Woodbridge Road, Menangle, NSW, Australia.

Australian Grains Genebank, Agriculture Victoria , Horsham, Victoria, Australia.

出版信息

Microbiol Spectr. 2023 Dec 12;11(6):e0266323. doi: 10.1128/spectrum.02663-23. Epub 2023 Oct 12.

DOI:10.1128/spectrum.02663-23
PMID:37823658
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10714761/
Abstract

Globally, viral diseases impair the growth and vigor of cultivated crops such as grains, leading to a significant reduction in quality, marketability, and competitiveness. As an island nation, Australia has a distinct advantage in using its border to prevent the introduction of damaging viruses, which threaten the continental agricultural sector. However, breeding programs in Australia rely on imported seeds as new sources of genetic diversity. As such, it is critical to remain vigilant in identifying new and emerging viral pathogens, by ensuring the availability of accurate genomic diagnostic tools at the grain biosecurity border. High-throughput sequencing offers game-changing opportunities in biosecurity routine testing. Genomic results are more accurate and informative compared to traditional molecular methods or biological indexing. The present work contributes to strengthening accurate phytosanitary screening, to safeguard the Australian grains industry, and expedite germplasm release to the end users.

摘要

在全球范围内,病毒疾病会削弱谷物等栽培作物的生长和活力,导致其质量、市场竞争力显著下降。作为一个岛国,澳大利亚利用边境来防止破坏性病毒的传入具有明显优势,这些病毒威胁着澳大利亚的大陆农业部门。然而,澳大利亚的育种计划依赖于进口种子作为新的遗传多样性来源。因此,至关重要的是,要通过确保在谷物生物安保边境提供准确的基因组诊断工具,保持对新出现的病毒性病原体的警惕。高通量测序为生物安保常规检测提供了改变游戏规则的机会。与传统的分子方法或生物指标相比,基因组结果更准确、更具信息量。本研究有助于加强准确的植物检疫筛选,以保护澳大利亚的谷物产业,并加速种质资源向最终用户的释放。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/047f/10714761/7156d08ff1a7/spectrum.02663-23.f002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/047f/10714761/874d0017ffe9/spectrum.02663-23.f001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/047f/10714761/7156d08ff1a7/spectrum.02663-23.f002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/047f/10714761/874d0017ffe9/spectrum.02663-23.f001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/047f/10714761/7156d08ff1a7/spectrum.02663-23.f002.jpg

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