Shum L, Jusko W J
J Chromatogr. 1986 Oct 31;382:207-13. doi: 10.1016/s0378-4347(00)83518-5.
A simple and selective high-performance liquid chromatographic procedure has been developed for the determination of verofylline in rat serum. This assay procedure, which is suitable for the analysis of microsamples (0.05 or 0.1 ml serum), involves a single extraction followed by isocratic chromatography on an RP-18 analytical column with ultraviolet detection at 280 nm. The calibration curve is linear within the study range 0.25-75 micrograms/ml. The intra-day and inter-day coefficients of variation were below 5.7%. The specificity of the assay was confirmed by comparing the lipophilicity, absorbance, and chromatographic property of the apparent and authentic verofylline.
已开发出一种简单且具选择性的高效液相色谱法，用于测定大鼠血清中的维罗非林。该检测方法适用于微量样品（0.05或0.1 ml血清）分析，包括单次萃取，然后在RP - 18分析柱上进行等度色谱分析，并于280 nm处进行紫外检测。校准曲线在0.25 - 75微克/毫升的研究范围内呈线性。日内和日间变异系数均低于5.7%。通过比较表观维罗非林和真实维罗非林的亲脂性、吸光度及色谱性质，证实了该检测方法的特异性。
