Jesse B W, Emery R S, Thomas J W
J Dairy Sci. 1986 Sep;69(9):2298-303. doi: 10.3168/jds.S0022-0302(86)80668-3.
Factors involved in regulation of bovine hepatic fatty acid oxidation were examined using liver slices. Fatty acid oxidation was measured as the conversion of 1-[14C] palmitate to 14CO2 and total [14C] acid-soluble metabolites. Extended (5 to 7 d) fasting of Holstein cows had relatively little effect on palmitate oxidation to acid-soluble metabolites by liver slices, although oxidation to CO2 was decreased. Feeding a restricted roughage, high concentrate ration to lactating cows resulted in inhibition of palmitate oxidation. Insulin, glucose, and acetate inhibited palmitate oxidation by bovine liver slices. We suggest the regulation of bovine hepatic fatty acid oxidation may be less dependent on hormonally induced alterations in enzyme activity as observed in rat liver and more dependent upon action of rumen fermentation products or their metabolites on enzyme systems involved in fatty acid oxidation.
利用肝切片研究了参与调节牛肝脏脂肪酸氧化的因素。脂肪酸氧化通过测量1-[14C]棕榈酸酯转化为14CO2以及总的[14C]酸溶性代谢产物来测定。荷斯坦奶牛长时间（5至7天）禁食对肝切片将棕榈酸酯氧化为酸溶性代谢产物的影响相对较小，尽管氧化为CO2的过程有所减少。给泌乳奶牛饲喂低粗饲料、高浓缩日粮会导致棕榈酸酯氧化受到抑制。胰岛素、葡萄糖和乙酸盐会抑制牛肝切片的棕榈酸酯氧化。我们认为，与在大鼠肝脏中观察到的情况相比，牛肝脏脂肪酸氧化的调节可能较少依赖于激素诱导的酶活性变化，而更多地依赖于瘤胃发酵产物或其代谢产物对参与脂肪酸氧化的酶系统的作用。
