Wiedermann C J, Goldman M E, Plutchok J J, Sertl K, Kaliner M A, Johnston-Early A, Cohen M H, Ruff M R, Pert C B
J Immunol. 1986 Dec 15;137(12):3928-32.
Bombesin found in neuroepithelial bodies and oat cell carcinoma of the lung, is thought to play an important role in normally developing and malignant lung. Monocytes-macrophages and human small cell lung carcinoma cells share several features, including macrophage-specific surface markers and the expression of functional receptors for bombesin-like neuropeptides and growth factors. Because small cell lung carcinoma cells synthesize immunoreactive bombesin, we investigated the possibility that alveolar macrophages also contain bombesin, a plausible hypothesis considering the many reports of neuropeptide production by immune cells and cells of bone marrow origin. Adherent human peripheral blood mononuclear cells as well as human and guinea pig alveolar macrophages were found to contain bombesin. The peptide was detected by radioimmunoassay, immunohistochemistry, high-pressure liquid chromatography with the use of different monospecific antibodies.
在神经上皮小体和肺燕麦细胞癌中发现的蛙皮素，被认为在正常发育的肺和恶性肺中发挥重要作用。单核细胞-巨噬细胞与人类小细胞肺癌细胞具有若干共同特征，包括巨噬细胞特异性表面标志物以及对蛙皮素样神经肽和生长因子功能性受体的表达。由于小细胞肺癌细胞能合成免疫反应性蛙皮素，我们研究了肺泡巨噬细胞也含有蛙皮素的可能性，鉴于免疫细胞和骨髓来源细胞产生神经肽的众多报道，这是一个合理的假设。发现贴壁的人外周血单核细胞以及人和豚鼠的肺泡巨噬细胞含有蛙皮素。使用不同的单特异性抗体，通过放射免疫测定、免疫组织化学、高压液相色谱法检测到了该肽。
