Sengoku K, Ishikawa M, Shibata S, Shimizu T
Nihon Sanka Fujinka Gakkai Zasshi. 1986 Oct;38(10):1727-32.
The intracellular oxygen tension in hamster embryos at various early stages of development was examined using a microelectrode technique. Changes in PO2 values before and after insemination in vitro were also investigated. The results were as follows. The oxygen tension in the culture medium was 83.8-99.0 mmHg. On the other hand, the mean oxygen tension in unfertilized oocytes was 51.1 +/- 7.3 mmHg (range 38.0-63.8 mmHg) and remained at these unfertilized levels throughout fertilization (49.6 +/- 7.1 mmHg). Slight but significant increases in oxygen tension in embryos occurred at the 2 cell stage and each successive stage. Mean values for PO2 in 2 cell, 4 cell and 6-8 cell stages were 58.5 +/- 5.6, 59.7 +/- 6.2 and 60.7 +/- 3.1 mmHg, respectively. These results suggested that the respiratory activity of oocytes seemed to be increased during the early cleavage stage and that this microelectrode technique should be useful in further studies of respiratory metabolism in oocytes and preimplantation embryos.
采用微电极技术检测了处于不同早期发育阶段的仓鼠胚胎的细胞内氧张力。还研究了体外受精前后PO2值的变化。结果如下。培养基中的氧张力为83.8 - 99.0 mmHg。另一方面，未受精卵母细胞的平均氧张力为51.1±7.3 mmHg（范围为38.0 - 63.8 mmHg），并且在整个受精过程中保持在这些未受精水平（49.6±7.1 mmHg）。胚胎在2细胞期及随后的每个阶段氧张力都有轻微但显著的增加。2细胞、4细胞和6 - 8细胞期的PO2平均值分别为58.5±5.6、59.7±6.2和60.7±3.1 mmHg。这些结果表明，卵母细胞的呼吸活性在早期卵裂阶段似乎有所增加，并且这种微电极技术在进一步研究卵母细胞和植入前胚胎的呼吸代谢方面应该是有用的。
