Department of Oral and Maxillofacial Surgery, Faculty of Dentistry, Chiang Mai University, Chiang Mai, Thailand.
Neurophysiology Unit, Cardiac Electrophysiology Research and Training Center, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand.
J Wound Care. 2023 Oct 2;32(10):676-684. doi: 10.12968/jowc.2023.32.10.676.
This study aimed to investigate the involvement of mitochondrial biogenesis, and determine the extent of fibroblast proliferation and cellular apoptosis, in the gingiva of patients who had undergone head and neck radiation, after receiving hyperbaric oxygen therapy (HBOT), in comparison with normal gingiva.
A total of 16 patients who had undergone head and neck radiation with HBOT and six healthy subjects were included in the study. After the completion of radiation therapy, patients received HBOT at 2 ATA for 90 minutes per session, and for 20 sessions per patient. Samples of gingival tissues were then taken. The levels of: transforming growth factor beta (TGF-β); phospho-nuclear factor kappa-light-chain-enhancer of activated B cells (p-NFϰB); nuclear factor kappa-light-chain-enhancer of activated B cells (NFϰB); proliferator-activated receptor gamma coactivator 1-alpha (PGC-1α); phospho-dynamin-related protein 1 at ser616 (p-Drp1); dynamin-related protein 1 (Drp1); Bcl-2-associated X-protein (Bax); and B-cell lymphoma 2 (Bcl-2) were determined using a Western blot. Independent t-test and Chi-squared tests were used in the study.
There were no differences in the levels of TGF-β, p-NFϰB, NFϰB, p-Drp1, Drp1, Bax and Bcl-2 between the two groups. However, the level of PGC-1α was greater in irradiated gingival tissues with HBOT than in the healthy gingiva.
Radiation-induced impaired wound healing can be improved by HBOT as indicated by levels of apoptosis, mitochondrial dynamics, cell proliferation and inflammation in irradiated gingiva with HBOT to a similar level to normal healthy gingiva. These findings may occur through an increase in mitochondrial biogenesis following HBOT.
本研究旨在探讨线粒体生物发生的参与情况，并确定接受高压氧治疗（HBOT）后头颈部放疗患者的牙龈中成纤维细胞增殖和细胞凋亡的程度，与正常牙龈相比。
共纳入 16 例接受头颈部放疗和 HBOT 的患者和 6 例健康受试者。放疗完成后，患者接受 2ATA 的 HBOT，每次 90 分钟，每位患者 20 次。然后取牙龈组织样本。使用 Western blot 测定转化生长因子β（TGF-β）；磷酸核因子 kappa-轻链增强子活化 B 细胞（p-NFκB）；核因子 kappa-轻链增强子活化 B 细胞（NFκB）；过氧化物酶体增殖物激活受体γ共激活因子 1-α（PGC-1α）；磷酸动力相关蛋白 1 丝氨酸 616（p-Drp1）；动力相关蛋白 1（Drp1）；B 细胞淋巴瘤 2 相关 X 蛋白（Bax）和 B 细胞淋巴瘤 2（Bcl-2）的水平。本研究采用独立 t 检验和卡方检验。
两组间 TGF-β、p-NFκB、NFκB、p-Drp1、Drp1、Bax 和 Bcl-2 水平无差异。然而，接受 HBOT 治疗的放射性牙龈组织中 PGC-1α 的水平高于健康牙龈。
HBOT 可改善辐射引起的伤口愈合受损，这表明接受 HBOT 治疗的放射性牙龈中细胞凋亡、线粒体动力学、细胞增殖和炎症的水平与正常健康牙龈相似。这些发现可能是通过 HBOT 后线粒体生物发生的增加而发生的。
