School of Chemistry and Life Sciences, Suzhou University of Science and Technology, No. 99 Xuefu Rd., Huqiu District, Suzhou, 215009, Jiangsu, People's Republic of China.
Biotechnol Lett. 2023 Dec;45(11-12):1529-1539. doi: 10.1007/s10529-023-03428-1. Epub 2023 Oct 13.
Erythritol is a valuable compound as sweetener and chemical material however cannot be fermented from the abundant substrate xylose.
The strain Trichosporonoides oedocephalis ATCC 16958 was employed to produce polyols including xylitol and erythritol by metabolic engineering approaches.
The introduction of a substrate-specific ribose-5-phosphate isomerase endowed T. oedocephalis with xylose-assimilation activity to produce xylitol, and eliminated glycerol production simultaneously. A more value-added product, erythritol was produced by further introducing a homologous xylulose kinase. The carbon flux was redirected from xylitol to erythritol by adding high osmotic pressure. The production of erythritol was improved to 46.5 g/L in flasks by fermentation adjustment, and the process was scaled up in a 5-L fermentor, with a 40 g/L erythritol production after 120 h, and a time-space yield of 0.56 g/L/h.
This study demonstrated the potential of T. oedocephalis in the synthesis of multiple useful products from xylose.
赤藓糖醇是一种有价值的化合物，可用作甜味剂和化学物质，但不能从丰富的木糖底物发酵得到。
采用代谢工程方法，利用异常威克汉姆酵母（Trichosporonoides oedocephalis）ATCC 16958 生产包括木糖醇和赤藓糖醇在内的多元醇。
引入一种底物特异性的核酮糖-5-磷酸异构酶，使异常威克汉姆酵母具有木糖同化活性，从而生产木糖醇，并同时消除甘油的产生。通过进一步引入同源木酮糖激酶，生产出更有价值的产品赤藓糖醇。通过添加高渗透压，将碳通量从木糖醇重新导向赤藓糖醇。通过发酵调整，将摇瓶中赤藓糖醇的产量提高到 46.5 g/L，在 5-L 发酵罐中放大规模后，经过 120 h 可生产 40 g/L 的赤藓糖醇，时空产率为 0.56 g/L/h。
本研究表明异常威克汉姆酵母具有从木糖合成多种有用产物的潜力。
