Department of Respiratory and Critical Care Medicine, The First Affiliated Hospital of Chongqing Medical University, Chongqing, 400016, PR China.
Chongqing Research Center for Pharmaceutical Engineering, College of Pharmacy, Chongqing Medical University, Chongqing, 400016, PR China.
Biosens Bioelectron. 2023 Dec 15;242:115734. doi: 10.1016/j.bios.2023.115734. Epub 2023 Oct 4.
Rapid and effective detection of Mycobacterium tuberculosis (MTB) is the crux of minimizing tuberculosis (TB) spread. Consequently, a new electrochemical aptasensor based on dual-signal output for ultrasensitive detection of MTB early secreted antigenic target 6 (ESAT-6) antigen was developed. Especially, a new nanocomposite MXene/CNPs/Au@Pt was synthesized for signal generation and amplification. In this biosensing architecture, dual independent signal outputs were achieved by coupling the electrochemical redox activity of fullerene nanoparticles (CNPs) with the effective electrocatalytic activity of Au@Pt nanoparticles. MXene possesses a large specific surface area, allowing densely loaded of these two electroactive materials, further improved sensing capability. In addition, specific ESAT-6 antigen binding aptamers were attached to Au@Pt to create the tracer label. With a typical sandwich format along with the introduction of the gold nanoparticle-loaded molybdenum disulfide (MoS-Au) as the sensing interface, the limit of detection (LOD) of the proposed aptasensor was 2.88 fg mL (DPV measurement) and 13.50 fg mL (IT measurement), respectively, with a broad linear range of 100 fg mL to 50 ng mL. Significantly, it exhibited better specificity and accuracy with a sensitivity of 97.5% and a specificity of 96.7% to distinguish healthy donors, other lung diseases and TB patients compared to commercial ELISA assay, holding a promising prospect in clinical diagnosis.
快速有效地检测结核分枝杆菌（MTB）是最大限度减少结核病（TB）传播的关键。因此，开发了一种新的基于双信号输出的电化学适体传感器，用于超灵敏检测 MTB 早期分泌抗原靶 6（ESAT-6）抗原。特别是，合成了一种新的纳米复合材料 MXene/CNPs/Au@Pt 用于信号产生和放大。在这种生物传感结构中，通过耦合富勒烯纳米粒子（CNPs）的电化学氧化还原活性和 Au@Pt 纳米粒子的有效电催化活性，实现了双独立信号输出。MXene 具有大的比表面积，允许这些两种电活性材料的密集负载，进一步提高了传感能力。此外，特定的 ESAT-6 抗原结合适体被连接到 Au@Pt 上以创建示踪标签。通过典型的三明治结构以及引入负载金纳米粒子的二硫化钼（MoS-Au）作为传感界面，所提出的适体传感器的检测限（LOD）分别为 2.88 fg mL（DPV 测量）和 13.50 fg mL（IT 测量），具有 100 fg mL 至 50 ng mL 的宽线性范围。与商业 ELISA 检测相比，该传感器对健康供体、其他肺部疾病和 TB 患者的区分具有 97.5%的灵敏度和 96.7%的特异性，显示出更好的特异性和准确性，在临床诊断中具有广阔的前景。
