Autophagy Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.
Colorectal Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.
Biochem Biophys Res Commun. 2023 Nov 19;682:281-292. doi: 10.1016/j.bbrc.2023.10.020. Epub 2023 Oct 5.
Covering surgical wounds with biomaterials, biologic scaffolds, and mesenchymal stem cells (MSCs) improves the healing process and reduces postoperative complications. This study was designed to evaluate and compare the effect of MSC-free/MSC-seeded new collagen/poly(3-hydroxybutyrate) (COL/P3HB) composite scaffold and human amniotic membrane (HAM) on the colon anastomosis healing process. COL/P3HB scaffold was prepared using freeze-drying method. MSCs were isolated and characterized from rat adipose tissue. After biocompatibility evaluation by MTT assay, MSCs were seeded on the scaffold and HAM by micro-mass seeding technique. In total, 35 male rats were randomly divided into five groups. After the surgical procedure, cecum incisions were covered by the MSC-free/MSC-seeded scaffold or HAM. Incisions in the control group were only sutured. One month later, the healing process was determined by stereological analysis. The Kruskal-Wallis followed by Dunn's tests were utilized for statistical outcome analysis (SPSS software version 21). COL/10% P3HB scaffold showed the best mechanical and structural properties (7.86 MPa strength, porosity more than 75%). MTT assay indicated that scaffold and especially HAM have suitable biocompatibility. Collagenization and neovascularization were significantly higher, and necrosis was considerably lower in all treated groups in comparison with the controls. MSC-seeded scaffold and HAM significantly decrease inflammation and increase gland volume compared with other groups. The MSC-seeded HAM was significantly successful in decreasing edema compared with other groups. Newly synthesized COL/P3HB scaffold improves the colon anastomosis healing; however, the major positive effect belonged to HAM. MSCs remarkably increase their healing process. Further investigations may contribute to confirming these results in other wound healing.
用生物材料、生物支架和间充质干细胞（MSCs）覆盖手术伤口可以改善愈合过程并减少术后并发症。本研究旨在评估和比较 MSC 无/MSC 种子新胶原/聚（3-羟基丁酸）（COL/P3HB）复合支架和人羊膜（HAM）对结肠吻合口愈合过程的影响。COL/P3HB 支架采用冷冻干燥法制备。从大鼠脂肪组织中分离和鉴定 MSC。通过 MTT 测定进行生物相容性评价后，通过微团播种技术将 MSC 接种到支架和 HAM 上。总共 35 只雄性大鼠被随机分为五组。手术后，用 MSC 无/MSC 种子支架或 HAM 覆盖肠切口。对照组的切口仅缝合。一个月后，通过体视学分析确定愈合过程。统计结果分析采用 Kruskal-Wallis 检验后 Dunn 检验（SPSS 软件版本 21）。COL/10%P3HB 支架具有最佳的机械和结构性能（7.86 MPa 强度，孔隙率超过 75%）。MTT 测定表明支架，特别是 HAM 具有良好的生物相容性。与对照组相比，所有治疗组的胶原化和新生血管形成明显增加，坏死明显减少。与其他组相比，MSC 接种支架和 HAM 明显减少炎症并增加腺体体积。与其他组相比，MSC 接种的 HAM 显著减少水肿。新合成的 COL/P3HB 支架可改善结肠吻合口愈合；然而，主要的积极影响属于 HAM。MSCs 显著增加了它们的愈合过程。进一步的研究可能有助于在其他伤口愈合中证实这些结果。
