Postgraduate Program in Animal Bioscience, Federal University of Jataí, GO, Brazil.
Instituto Nacional de Investigación Agropecuaria, Estación Experimental INIA La Estanzuela, Colonia, Uruguay.
Reprod Biol. 2023 Dec;23(4):100813. doi: 10.1016/j.repbio.2023.100813. Epub 2023 Oct 11.
Cystic endometrial hyperplasia (CEH)-pyometra syndrome is the most common uterine pathological condition reported in breeding bitches, however, their described effects on fertility are limited to uterine disorders and conception rates. As the preantral follicle population represents the available reserve of gametes recruited during the lifespan, the aim of this study was to evaluate the effects of CEH-pyometra syndrome on the: (i) preantral follicle morphology, (ii) developing follicle rates, and (iii) preantral follicle and stromal cell densities. Ovarian fragments from bitches subjected to elective or therapeutic ovariohysterectomy were allocated according to uterine diagnosis as follows: control (n = 7, clinically healthy), CEH-mucometra (n = 8, uterine lumen filled with a sterile mucus), and pyometra (n = 17, presence of a purulent mucus) groups. Overall, the control group had 3.4 and 4.1-fold higher probability (P < 0.0001) of the presence of normal preantral follicles compared with CEH-mucometra and pyometra groups, respectively. Moreover, ovarian fragments from the pyometra group showed an increase in the percentage of developing follicles (P < 0.05) compared to the control. Both CEH-mucometra and pyometra groups showed lower (P < 0.05) preantral follicle and stromal cell densities (P < 0.05) compared to the control. In summary, the CEH-pyometra syndrome decreased the percentage of morphologically normal follicles and enhanced the developing follicle rates. Additionally, a reduction of preantral follicle and stromal cell densities suggests that the inappropriate uterine environment induced by CEH-pyometra syndrome can lead to premature depletion of ovarian reserve.
囊性子宫内膜增生（CEH）-子宫积脓综合征是繁殖犬中最常见的子宫病理状况，但它们对生育力的描述仅限于子宫疾病和受孕率。由于窦前卵泡群代表了一生中募集的可用配子储备，因此本研究旨在评估 CEH-子宫积脓综合征对以下方面的影响：（i）窦前卵泡形态，（ii）发育卵泡率，和（iii）窦前卵泡和基质细胞密度。根据子宫诊断，将接受选择性或治疗性卵巢子宫切除术的犬的卵巢碎片分配如下：对照组（n=7，临床健康）、CEH-黏液性子宫积脓组（n=8，子宫腔充满无菌黏液）和子宫积脓组（n=17，存在化脓性黏液）。总体而言，与 CEH-黏液性子宫积脓组和子宫积脓组相比，对照组正常窦前卵泡存在的可能性分别高出 3.4 倍和 4.1 倍（P<0.0001）。此外，与对照组相比，子宫积脓组的发育卵泡百分比增加（P<0.05）。CEH-黏液性子宫积脓组和子宫积脓组的窦前卵泡和基质细胞密度均较低（P<0.05），与对照组相比。总之，CEH-子宫积脓综合征降低了形态正常卵泡的百分比，并增加了发育卵泡的比例。此外，窦前卵泡和基质细胞密度的降低表明，CEH-子宫积脓综合征引起的不适当子宫环境可导致卵巢储备过早耗竭。
