School of Environmental Studies, China University of Geosciences (Wuhan), Wuhan 430074, China.
Chongqing 136 Geology and Mineral Resources Co. LTD, China.
J Hazard Mater. 2024 Jan 15;462:132676. doi: 10.1016/j.jhazmat.2023.132676. Epub 2023 Sep 29.
Antimony (Sb) is a toxic metalloid widely distributed in the natural environments. Microorganisms, especially fungi, could serve as ideal biomaterials for bioremediation of Sb-polluted soils and waters. In this study, we isolated an antimony-resistant fungus, Sarocladium kiliense ZJ-1, from a slag sample collected in Xikuangshan Sb mine in P. R. China. ZJ-1 showed an extremely high resistance to Sb, with a MIC level of > 175 mM for arsenite [Sb(Ⅲ)] and 40 mM for arsenate [Sb(V)]. Whole genomic analysis identified multiple Sb (Ⅲ)- and/or As(Ⅲ)-resistant genes on ZJ-1's genome, which may partially explain its hyper-resistance to Sb. The potential of ZJ-1 in removing Sb from Sb(Ⅲ) or Sb(V) solutions was also quantified. The average biosorption capacity of ZJ-1 for Sb(Ⅲ) and Sb(V) is 635.14 mg/g and 149.65 mg/g, respectively, in Sb aqueous solutions with an initial concentration of 2000 mg/L (16.43 mM). Besides, almost 99% of Sb(Ⅲ) in the growing system was removed with an initial concentration of 500 mg/L (4.11 mM). Furthermore, Fourier transformation infrared spectroscopy (FT-IR) and X-ray photoelectron spectroscopy (XPS) were used to probe the Sb adsorption mechanism on ZJ-1, and -OH, -NH, -COOH, C-O and C-O-C were found to be the main surface functional groups of ZJ-1 cells to adsorb Sb.
锑（Sb）是一种广泛分布于自然环境中的有毒类金属。微生物，尤其是真菌，可以作为生物修复 Sb 污染土壤和水体的理想生物材料。本研究从中国锡矿山 Sb 矿的炉渣样品中分离到一株抗 Sb 真菌Sarocladium kiliense ZJ-1。ZJ-1 对 Sb 具有极高的抗性，亚砷酸盐[Sb（Ⅲ）]和砷酸盐[Sb（V）]的 MIC 水平分别>175 mM 和 40 mM。全基因组分析鉴定出 ZJ-1 基因组上存在多个 Sb（Ⅲ）和/或 As（Ⅲ）抗性基因，这可能部分解释了其对 Sb 的超抗性。还定量评估了 ZJ-1 从 Sb（Ⅲ）或 Sb（V）溶液中去除 Sb 的潜力。在 Sb 初始浓度为 2000 mg/L（16.43 mM）的水溶液中，ZJ-1 对 Sb（Ⅲ）和 Sb（V）的平均生物吸附容量分别为 635.14 和 149.65 mg/g。此外，在 Sb 初始浓度为 500 mg/L（4.11 mM）的生长体系中，几乎 99%的 Sb（Ⅲ）被去除。此外，傅里叶变换红外光谱（FT-IR）和 X 射线光电子能谱（XPS）用于探测 ZJ-1 上 Sb 的吸附机制，发现 -OH、-NH、-COOH、C-O 和 C-O-C 是 ZJ-1 细胞吸附 Sb 的主要表面官能团。
