Centre for Water Resources Studies, Department of Civil and Resource Engineering, Dalhousie University, 1360 Barrington Street, Halifax, NS, B3H 4R2, Canada.
Sci Rep. 2023 Oct 13;13(1):17336. doi: 10.1038/s41598-023-44528-2.
Human viruses pose a significant health risk in freshwater environments, but current monitoring methods are inadequate for detecting viral presence efficiently. We evaluated a novel passive in-situ concentration method using granular activated carbon (GAC). This study detected and quantified eight enteric and non-enteric, pathogenic viruses in a freshwater recreational lake in paired grab and GAC passive samples. The results found that GAC passive sampling had a higher detection rate for all viruses compared to grab samples, with adenovirus found to be the most prevalent virus, followed by respiratory syncytial virus, norovirus, enterovirus, influenza A, SARS-CoV-2, and rotavirus. GAC in-situ concentration allowed for the capture and recovery of viral gene copy targets that ranged from one to three orders of magnitude higher than conventional ex-situ concentration methods used in viral monitoring. This simple and affordable sampling method may have far-reaching implications for reducing barriers associated with viral monitoring across various environmental contexts.
人类病毒在淡水环境中构成重大健康风险，但当前的监测方法在有效检测病毒存在方面还不够完善。我们评估了一种使用颗粒状活性炭（GAC）的新型被动原位浓缩方法。本研究在一对 Grab 和 GAC 被动样本中检测并定量了淡水娱乐湖中八种肠内和非肠内、致病性病毒。结果表明，与 Grab 样本相比，GAC 被动采样对所有病毒的检测率更高，其中腺病毒是最常见的病毒，其次是呼吸道合胞病毒、诺如病毒、肠道病毒、流感 A、SARS-CoV-2 和轮状病毒。GAC 原位浓缩允许捕获和回收病毒基因拷贝目标，其范围比病毒监测中使用的传统原位浓缩方法高出一到三个数量级。这种简单且经济实惠的采样方法可能会对降低与各种环境背景下的病毒监测相关的障碍产生深远影响。
