Hayes Emalie K, Sweeney Crystal L, Fuller Megan, Erjavec Genevieve B, Stoddart Amina K, Gagnon Graham A
Centre for Water Resources Studies, Faculty of Engineering, Dalhousie University, Halifax, NS B3H 4R2, Canada.
ACS ES T Water. 2022 Jan 21;2(11):1910-1920. doi: 10.1021/acsestwater.1c00441. eCollection 2022 Nov 11.
In developing an effective monitoring program for the wastewater surveillance of SARS-CoV-2 ribonucleic acid (RNA), the importance of sampling methodology is paramount. Passive sampling has been shown to be an effective tool to detect SARS-CoV-2 RNA in wastewater. However, the adsorption characteristics of SARS-CoV-2 RNA on passive sampling material are not well-understood, which further obscures the relationship between wastewater surveillance and community infection. In this work, adsorption kinetics and equilibrium characteristics were evaluated using batch-adsorption experiments for heat-inactivated SARS-CoV-2 (HI-SCV-2) adsorption to electronegative filters. Equilibrium isotherms were assessed or a range of total suspended solids (TSS) concentrations (118, 265, and 497 mg L) in wastewater, and a modeled of 7 × 10 GU cm was found. Surrogate adsorption kinetics followed a pseudo-first-order model in wastewater with maximum concentrations achieved within 24 h. In both field and isotherm experiments, equilibrium behavior and viral recovery were found to be associated with wastewater and eluate TSS. On the basis of the results of this study, we recommend a standard deployment duration of 24-48 h and the inclusion of eluate TSS measurement to assess the likelihood of solids inhibition during analysis.
在制定一个有效的监测计划用于对严重急性呼吸综合征冠状病毒2（SARS-CoV-2）核糖核酸（RNA）进行废水监测时，采样方法的重要性至关重要。被动采样已被证明是检测废水中SARS-CoV-2 RNA的有效工具。然而，SARS-CoV-2 RNA在被动采样材料上的吸附特性尚未得到充分了解，这进一步模糊了废水监测与社区感染之间的关系。在这项工作中，通过批量吸附实验评估了热灭活SARS-CoV-2（HI-SCV-2）对带负电滤膜的吸附动力学和平衡特性。评估了废水在一系列总悬浮固体（TSS）浓度（118、265和497 mg/L）下的平衡等温线，发现模拟的浓度为7×10 GU/cm。替代吸附动力学在废水中遵循准一级模型，在24小时内达到最大浓度。在现场和等温线实验中，均发现平衡行为和病毒回收率与废水和洗脱液中的TSS有关。基于本研究结果，我们建议标准部署持续时间为24 - 48小时，并纳入洗脱液TSS测量以评估分析过程中固体抑制的可能性。
