Hanani M, Lernau O Z, Zamir O, Nissan S
J Pediatr Surg. 1986 Oct;21(10):848-51. doi: 10.1016/s0022-3468(86)80005-7.
The activity of isolated muscle strips from normal and aganglionic human large bowel was studied in vitro. The intrinsic nerves were stimulated electrically and by nicotinic agonists. The ganglionic preparations displayed a strong inhibitory response due to the release of both norepinephrine and a noncholinergic, nonadrenergic inhibitory neurotransmitter. In the aganglionic strips (obtained from patients with Hirschsprung's disease), nerve activation tended to evoke contraction, apparently due to enhancement in the release of acetylcholine. At the same time, the release of norepinephrine appeared to be less than normal. A particularly interesting finding in the aganglionic muscle strips was the presence of a substantial inhibitory response due to the release of a noncholinergic, nonadrenergic substance. These results provide further evidence for the importance of the innervation of the aganglionic segment in Hirschsprung's disease.
在体外研究了来自正常和无神经节的人大肠的离体肌条的活性。对内在神经进行电刺激和烟碱样激动剂刺激。神经节制剂由于去甲肾上腺素和一种非胆碱能、非肾上腺素能抑制性神经递质的释放而表现出强烈的抑制反应。在无神经节肌条（取自患有先天性巨结肠症的患者）中，神经激活往往会引起收缩，显然是由于乙酰胆碱释放增加所致。同时，去甲肾上腺素的释放似乎低于正常水平。在无神经节肌条中一个特别有趣的发现是，由于一种非胆碱能、非肾上腺素能物质的释放而出现了显著的抑制反应。这些结果为先天性巨结肠症中无神经节段神经支配的重要性提供了进一步的证据。
