Department of Neurosurgery, CHA Bundang Medical Center, CHA University, 59 Yatap-ro, Bundang-gu, Seongnam-si 13496, Gyeonggi-do, Republic of Korea.
Department of Life Science, CHA University, Boondagger, Seongnam-si 13493, Gyeonggi-do, Republic of Korea.
Int J Mol Sci. 2023 Sep 26;24(19):14589. doi: 10.3390/ijms241914589.
The purpose of this study was to investigate the anti-inflammatory effect of tegoprazan (TEGO) in lipopolysaccharide (LPS)-stimulated bone-marrow-derived macrophages (BMMs). To this end, compared to methylprednisolone (MP; positive control), we evaluated whether TEGO effectively differentiates LPS-stimulated BMMs into M2-phenotype macrophages. Moreover, the expression of pro- and anti-inflammatory cytokines genes influenced by TEGO was measured using quantitative real-time polymerase chain reaction (qRT-PCR) analysis. TEGO was found to reduce nitric oxide (NO) production in BMMs significantly. In addition, TEGO significantly decreased and increased the gene expression levels of pro-inflammatory and anti-inflammatory cytokines, respectively. In addition, we evaluated the phosphorylated values of the extracellular signal-regulatory kinase (ERK) and p38 in the mitogen-activated protein (MAP) kinase signaling pathway through Western blotting. TEGO significantly reduced the phosphorylated values of the ERK and p38. In other words, TEGO suppressed the various pro-inflammatory responses in LPS-induced BMMs. These results show that TEGO has the potential to be used as an anti-inflammatory agent.
本研究旨在探讨替戈拉赞（TEGO）在脂多糖（LPS）刺激的骨髓来源巨噬细胞（BMM）中的抗炎作用。为此，与甲泼尼龙（MP；阳性对照）相比，我们评估了 TEGO 是否能有效将 LPS 刺激的 BMM 分化为 M2 表型巨噬细胞。此外，还通过定量实时聚合酶链反应（qRT-PCR）分析测量了 TEGO 影响的促炎和抗炎细胞因子基因的表达。结果发现 TEGO 可显著减少 BMM 中一氧化氮（NO）的产生。此外，TEGO 还分别显著降低和增加了促炎和抗炎细胞因子的基因表达水平。此外，我们还通过 Western blot 评估了 MAP 激酶信号通路中细胞外信号调节激酶（ERK）和 p38 的磷酸化值。TEGO 显著降低了 ERK 和 p38 的磷酸化值。换句话说，TEGO 抑制了 LPS 诱导的 BMM 中的各种促炎反应。这些结果表明，TEGO 有可能被用作抗炎剂。
Zotero 插件
