Research Center of Biochemistry and Advanced Molecular Biology, Department of Experimental and Clinical Medicine, "Magna Graecia" University of Catanzaro, 88100 Catanzaro, Italy.
Laboratory of Molecular Haematopoiesis and Stem Cell Biology, Department of Experimental and Clinical Medicine, University Magna Græcia, 88100 Catanzaro, Italy.
Int J Mol Sci. 2023 Sep 29;24(19):14755. doi: 10.3390/ijms241914755.
The human zinc finger protein 521 (ZNF521) is a co-transcriptional factor with multiple recognized regulatory functions in a range of normal, cancer and stem cell compartments. ZNF521 regulates proliferation, progression and CSC (cancer stem cell) compartments in human ovarian cancer (hOC), which is a very aggressive and late-diagnosed female tumor. Two other important regulators of hOC are the NRF2 and NOTCH signaling pathways. In the present paper, the mRNA and protein levels of ZNF521 were correlated with those of the NRF2-NOTCH signaling components in two different hOC cell lines and in a public dataset of 381 hOC patients. The data show that high levels of ZNF521 significantly increase NRF2-NOTCH signaling expression; conversely, the silencing of ZNF521 impairs NRF2-NOTCH signaling. This experimental work shows that, in hOC, different levels of ZNF521 modulate the NRF2-NOTCH signaling pathway and also influences hOC CSC properties.
人类锌指蛋白 521（ZNF521）是一种共转录因子，在多种正常、癌症和干细胞区室中具有多种公认的调节功能。ZNF521 调节人卵巢癌（hOC）的增殖、进展和 CSC（癌症干细胞）区室，hOC 是一种非常侵袭性和晚期诊断的女性肿瘤。NRF2 和 NOTCH 信号通路是另外两个调节 hOC 的重要调控因子。在本论文中，在两种不同的 hOC 细胞系和 381 名 hOC 患者的公共数据集上，将 ZNF521 的 mRNA 和蛋白水平与 NRF2-NOTCH 信号成分的水平进行了关联。数据表明，高水平的 ZNF521 显著增加了 NRF2-NOTCH 信号表达；相反，ZNF521 的沉默会损害 NRF2-NOTCH 信号。这项实验工作表明，在 hOC 中，不同水平的 ZNF521 调节 NRF2-NOTCH 信号通路，并影响 hOC CSC 特性。
