Garau J M, di Paola G R, Charreau E H
J Reprod Med. 1986 Oct;31(10):987-91.
Seventy-seven samples of healthy and diseased vulvar epithelium (one per patient) were processed using the carbon-coated-dextran biochemical technique. The results were analyzed according to Scatchard or the unique point technique. The samples of normal tissue were obtained from patients who underwent surgical procedures for different gynecologic pathologies. The samples of diseased tissue were obtained with punch biopsy from patients with several vulvar disorders. Ten samples were obtained from the episiotomy wound in pregnant patients during labor. Estrogen receptors were assayed in all cases. Progesterone receptors were assayed simultaneously in 73 of the cases. The minimum concentration considered positive was 2 fmol/mg of protein. A map of the vulvar region was drawn, and samples were obtained from different sites whenever possible.
采用碳包被葡聚糖生化技术处理77份健康和患病外阴上皮样本（每位患者1份）。根据Scatchard法或独特点技术对结果进行分析。正常组织样本取自因不同妇科疾病接受外科手术的患者。患病组织样本通过对患有多种外阴疾病的患者进行打孔活检获得。10份样本取自分娩期孕妇的会阴切开伤口。所有病例均检测雌激素受体。73例同时检测孕激素受体。将最低阳性浓度设定为2 fmol/mg蛋白质。绘制了外阴区域图，并尽可能从不同部位获取样本。
