Zhang Junjie, Wang Nan, Li Shuo, Wang Jingqi, Feng Yufeng, Wang Entao, Li Youguo, Yang Tao, Chen Wenfeng
College of Food and Bioengineering, Zhengzhou University of Light Industry, Zhengzhou 450002, China.
Collaborative Innovation Center for Food Production and Safety of Henan Province, Zhengzhou 450002, China.
Plants (Basel). 2023 Sep 28;12(19):3421. doi: 10.3390/plants12193421.
Chickpea ( L.) is currently the third most important legume crop in the world. It could form root nodules with its symbiotic rhizobia in soils and perform bio-nitrogen fixation. is a prevalent species in the world, except China, where is the main species associated with chickpea. There were significant differences in the competitive ability between and in sterilized and unsterilized soils collected from Xinjiang, China, where chickpea has been grown long term. In unsterilized soils, was more competitive than , while in sterilized soils, the opposite was the case. In addition, the competitive ability of in soils of new areas of chickpea cultivation was significantly higher than that of . It was speculated that there might be some biological factors in Xinjiang soils of China that could differentially affect the competitive nodulation of these two chickpea rhizobia. To address this question, we compared the composition and diversity of microorganisms in the rhizosphere of chickpea inoculated separately with the above two rhizobial species in soils from old and new chickpea-producing regions.
Chickpea rhizosphere microbial diversity and composition varied in different areas and were affected significantly due to rhizobial inoculation. In general, eight dominant phyla with 34 dominant genera and 10 dominant phyla with 47 dominant genera were detected in the rhizosphere of chickpea grown in soils of Xinjiang and of the new zones, respectively, with the inoculated rhizobia. Proteobacteria and Actinobacteria were dominant at the phylum level in the rhizosphere of all soils. appeared significantly enriched after inoculation with in soils from Xinjiang, a phenomenon not found in the new areas of chickpea cultivation, demonstrating that might be the key biological factor affecting the competitive colonization of and there.
Different chickpea rhizobial inoculations of and affected the rhizosphere microbial composition in different sampling soils from different chickpea planting areas. Through high throughput sequencing and statistical analysis, it could be found that might be the key microorganism influencing the competitive nodulation of different chickpea rhizobia in different soils, as it is the dominant non-rhizobia community in Xinjiang rhizosphere soils, but not in other areas.
鹰嘴豆（Cicer arietinum L.）是目前世界上第三重要的豆类作物。它能与土壤中的共生根瘤菌形成根瘤并进行生物固氮。除中国外，Rhizobium leguminosarum是世界上普遍存在的物种，而在中国，Mesorhizobium ciceri是与鹰嘴豆相关的主要物种。在中国新疆长期种植鹰嘴豆的地区，从灭菌和未灭菌土壤中采集的Rhizobium leguminosarum和Mesorhizobium ciceri的竞争能力存在显著差异。在未灭菌土壤中，Rhizobium leguminosarum比Mesorhizobium ciceri更具竞争力，而在灭菌土壤中则相反。此外，在鹰嘴豆新种植区土壤中，Rhizobium leguminosarum的竞争能力明显高于Mesorhizobium ciceri。据推测，中国新疆土壤中可能存在一些生物因素，会对这两种鹰嘴豆根瘤菌的竞争结瘤产生不同影响。为解决这个问题，我们比较了在鹰嘴豆老产区和新产区土壤中分别接种上述两种根瘤菌的鹰嘴豆根际微生物的组成和多样性。
鹰嘴豆根际微生物多样性和组成在不同地区有所不同，并受到根瘤菌接种的显著影响。总体而言，在新疆土壤和新种植区土壤中生长的接种了根瘤菌的鹰嘴豆根际，分别检测到8个优势门、34个优势属和10个优势门、47个优势属。在所有土壤的根际中，变形菌门和放线菌门在门水平上占主导地位。在新疆土壤中接种Rhizobium leguminosarum后，Mesorhizobium ciceri显著富集，而在鹰嘴豆新种植区未发现这种现象，这表明Mesorhizobium ciceri可能是影响Rhizobium leguminosarum和Mesorhizobium ciceri在那里竞争定殖的关键生物因素。
不同的鹰嘴豆根瘤菌Rhizobium leguminosarum和Mesorhizobium ciceri接种影响了来自不同鹰嘴豆种植区的不同采样土壤中的根际微生物组成。通过高通量测序和统计分析可以发现 Mesorhizobium ciceri可能是影响不同土壤中不同鹰嘴豆根瘤菌竞争结瘤的关键微生物，因为它是新疆根际土壤中的优势非根瘤菌群落，而在其他地区则不是。
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