Terletskaya Nina V, Khapilina Oxana N, Turzhanova Ainur S, Erbay Malika, Magzumova Saule, Mamirova Aigerim
Faculty of Biology and Biotechnology, Al-Farabi Kazakh National University, Al-Farabi 71, Almaty 050040, Kazakhstan.
Institute of Genetic and Physiology, Al-Farabi 93, Almaty 050040, Kazakhstan.
Plants (Basel). 2023 Oct 3;12(19):3470. doi: 10.3390/plants12193470.
The adaptive potential and biochemical properties of the species make them promising for introduction into agriculture and markets, particularly in arid conditions. Molecular genetic polymorphism analysis is the most powerful tool for studying plant resources; therefore, the current study aimed to investigate the polymorphisms of allelic variations in the and gene families, as well as the genetic diversity of six species, using retrotransposon-based fingerprinting with the multi-locus EPIC-PCR profiling approach. Additionally, the iPBS PCR amplification was employed for genome profiling, revealing variations in genetic diversity among the studied samples. The observed genetic diversity in species contributes to their enhanced tolerance to adverse environmental conditions. The knowledge about the genetic diversity of genes crucial in plant development and stress resistance can be useful for the genetic improvement of cultivated species.
该物种的适应潜力和生化特性使其有望被引入农业和市场,特别是在干旱条件下。分子遗传多态性分析是研究植物资源最有力的工具;因此,本研究旨在利用基于反转录转座子的指纹图谱和多位点EPIC-PCR分析方法,研究该基因家族中等位基因变异的多态性,以及六个该物种的遗传多样性。此外,采用iPBS PCR扩增进行基因组分析,揭示了所研究的该样本间遗传多样性的差异。在所观察到的该物种遗传多样性有助于其增强对不利环境条件的耐受性。有关植物发育和抗逆性关键基因的遗传多样性知识,可用于栽培该物种的遗传改良。