Laboratory of Electrochemistry and PATLAB, National Institute of Research for Electrochemistry and Condensed Matter, 202 Splaiul Independentei Str., 060021 Bucharest, Romania.
Faculty of Chemical Engineering and Biotechnologies, National University of Science & Technology Politehnica Bucharest, 060021 Bucharest, Romania.
Sensors (Basel). 2023 Sep 23;23(19):8046. doi: 10.3390/s23198046.
Two-needle 3D stochastic microsensors based on boron- and nitrogen-decorated gra-phenes, modified with N-(2-mercapto-1H-benzo[d]imidazole-5-yl), were designed and used for the molecular recognition and quantification of CA 72-4, CA 19-9, CEA and CA 125 biomarkers in biological samples such as whole blood, urine, saliva and tumoral tissue. The NBGr-2 sensor yielded lower limits of determination. For CEA, the LOD was 4.10 × 10 s g mL, while for CA72-4, the LOD was 4.00 × 10 s U mL. When the NBGr-1 sensor was employed, the best results were obtained for CA12-5 and CA19-9, with values of LODs of 8.37 × 10 s U mL and 2.09 × 10 s U mL, respectively. High sensitivities were obtained when both sensors were employed. Broad linear concentration ranges favored their determination from very low to higher concentrations in biological samples, ranging from 8.37 × 10 to 8.37 × 10 s U mL for CA12-5 when using the NBGr-1 sensor, and from 4.10 × 10 to 2.00 × 10 s g mL for CEA when using the NBGr-2 sensor. Student's -test showed that there was no significant difference between the results obtained utilizing the two microsensors for the screening tests, at a 99% confidence level, with the results obtained being lower than the tabulated values.
基于硼和氮掺杂的 gra-phenes 的双针 3D 随机微传感器，经 N-(2-巯基-1H-苯并[d]咪唑-5-基)修饰，用于识别和定量生物样品中的 CA 72-4、CA 19-9、CEA 和 CA 125 生物标志物，如全血、尿液、唾液和肿瘤组织。NBGr-2 传感器的测定下限更低。对于 CEA，LOD 为 4.10×10 s g mL，而对于 CA72-4，LOD 为 4.00×10 s U mL。当使用 NBGr-1 传感器时，CA12-5 和 CA19-9 获得了最佳结果，LOD 值分别为 8.37×10 s U mL 和 2.09×10 s U mL。当使用两个传感器时，都获得了很高的灵敏度。宽线性浓度范围有利于从非常低到较高浓度的生物样品中进行测定，当使用 NBGr-1 传感器时，CA12-5 的浓度范围为 8.37×10 至 8.37×10 s U mL，当使用 NBGr-2 传感器时，CEA 的浓度范围为 4.10×10 至 2.00×10 s g mL。学生 t 检验表明，在 99%置信水平下，两种微传感器的筛选测试结果没有显著差异，结果低于表列值。
