牛胰原羧肽酶A-S6三元复合物亚基III的结晶及初步X射线研究

Crystallization and preliminary X-ray study of subunit III of the bovine pancreatic procarboxypeptidase A-S6 ternary complex.

作者信息

Cambillau C, Kerfelec B, Foglizzo E, Chapus C

出版信息

J Mol Biol. 1986 Jun 20;189(4):709-10. doi: 10.1016/0022-2836(86)90500-0.

Abstract

Subunit III of the bovine pancreatic procarboxypeptidase A-S6 ternary complex was dissociated from the complex, purified and crystallized using the hanging- or sitting-drop method of vapour diffusion, with ammonium sulphate as the precipitant. The assays were carried out at pH 4.2 (20 mM-acetate buffer). An X-ray examination of the crystals shows that they are monoclinic, with a space group P21 and cell dimensions a = 47.9 A, b = 61.3 A, c = 39.0 A and beta = 95.0 degrees. The asymmetric unit contains one molecule of 25,800 Mr. The crystals are suitable for structure determination to at least 2.8 A resolution.

摘要

牛胰原羧肽酶A-S6三元复合物的亚基III从复合物中解离出来，进行纯化，并采用气相扩散的悬滴或坐滴法，以硫酸铵作为沉淀剂进行结晶。测定在pH 4.2（20 mM乙酸盐缓冲液）条件下进行。对晶体的X射线检查表明，它们是单斜晶系，空间群为P21，晶胞参数a = 47.9 Å，b = 61.3 Å，c = 39.0 Å，β = 95.0°。不对称单元包含一个分子量为25,800的分子。这些晶体适合进行结构测定，分辨率至少可达2.8 Å。