Aruguete Deborah M, Wyckoff Alaina, David Savanna, Steves Shannon, Crissman Riley, McCaleb Lydia
Department of Environmental Science, Penn State Behrend, 4205 College Drive, Erie, PA, 16563, USA.
Department of Biology, Penn State Behrend, 4205 College Drive, Erie, PA, 16563, USA.
Chemosphere. 2023 Dec;345:140442. doi: 10.1016/j.chemosphere.2023.140442. Epub 2023 Oct 13.
The growth effects and tissue accumulation of palladium (Pd) in the ubiquitous white-rot macrofungus Pleurotus ostreatus (oyster mushroom) are reported. Submerged cultures of P. ostreatus were exposed to Pd (as NaPdCl) at concentrations of 0, 6.25, 12.5, 25, 50 and 100 mg/L in potato dextrose broth media and incubated for 18 days at 25 °C. The growth response was measured as dried tissue biomass. Relative to controls, growth was partially inhibited at [Pd] = 25 and 50 mg/L. The minimum inhibitory concentration (MIC) of Pd was 100 mg Pd/L. Mean Pd concentrations of dry tissue (± standard error) ranged from 93.5 ± 17.1 mg Pd/kg to 1912.0 ± 293.9 mg Pd/kg, with bioconcentration factors (BCFs) ranging from 16.10 ± 4.17 to 40.91 ± 8.89. A linear positive log-log relationship was found between the C and [Pd] (R = 0.476), consistent with a Freundlich isotherm model of sorption. This relationship suggested that physicochemical processes may dominate tissue Pd accumulation in this system rather than biological processes.
本文报道了普遍存在的白腐大型真菌平菇（糙皮侧耳）中钯（Pd）的生长效应和组织积累情况。将平菇的液体培养物在马铃薯葡萄糖肉汤培养基中分别暴露于浓度为0、6.25、12.5、25、50和100 mg/L的钯（以NaPdCl形式），并在25℃下培养18天。以干燥组织生物量来衡量生长反应。相对于对照，在钯浓度为25和50 mg/L时生长受到部分抑制。钯的最低抑菌浓度（MIC）为100 mg Pd/L。干组织中的平均钯浓度（±标准误差）范围为93.5±17.1 mg Pd/kg至1912.0±293.9 mg Pd/kg，生物富集系数（BCF）范围为16.10±4.17至40.91±8.89。在浓度（C）和钯浓度（[Pd]）之间发现了线性正对数-对数关系（R = 0.476），这与弗伦德利希吸附等温线模型一致。这种关系表明，在该系统中，物理化学过程可能主导组织中钯的积累，而非生物过程。
