Alberta Precision Laboratories, Public Health Laboratory, Edmonton, AB, Canada.
Department of Laboratory Medicine and Pathology, University of Alberta, Edmonton, AB, Canada.
Sci Rep. 2023 Oct 14;13(1):17478. doi: 10.1038/s41598-023-44796-y.
Omicron has become the dominant SARS-CoV-2 variant globally since December 2021, with distinct waves being associated with separate Omicron sublineages. Rapid detection of BA.1, BA.2, BA.4, and BA.5 was accomplished in the province of Alberta, Canada, through the design and implementation of real-time reverse transcriptase PCR assays targeting S:N501Y, S:ins214EPE, S:H69/V70, ORF7b:L11F, and M:D3N. Using the combination of results for each of these markers, samples could be designated as belonging to sublineages within BA.1, BA.2, BA.4, or BA.5. The analytical sensitivity of these markers ranged from 132 to 2229 copies/mL and in-laboratory accuracy was 98.9-100%. A 97.3% agreement using 12,592 specimens was demonstrated for the assays compared to genome sequencing. The use of these assays, combined with genome sequencing, facilitated the surveillance of SARS-CoV-2 lineages throughout a BA.5-dominated period.
奥密克戎（Omicron）自 2021 年 12 月以来已成为全球 SARS-CoV-2 的主要变体，与单独的奥密克戎亚谱系相关的不同波次。通过设计和实施针对 S:N501Y、S:ins214EPE、S:H69/V70、ORF7b:L11F 和 M:D3N 的实时逆转录酶 PCR 检测，在加拿大阿尔伯塔省实现了对 BA.1、BA.2、BA.4 和 BA.5 的快速检测。利用每个标记的结果组合，可以将样本指定为属于 BA.1、BA.2、BA.4 或 BA.5 亚谱系。这些标记的分析灵敏度范围为 132 至 2229 拷贝/ml，实验室内部准确性为 98.9-100%。与基因组测序相比，这些检测方法在 12592 个样本中的一致性为 97.3%。这些检测方法的使用，结合基因组测序，有助于在 BA.5 主导时期对 SARS-CoV-2 谱系进行监测。
