[ODF2基因敲低对雄性小鼠精子活力和生育能力的影响]
[Impacts of ODF2 gene knockdown on the sperm motility and fertility of male mice].
作者信息
Lü Zi-Li, Shan Xu-Dong, DU Juan, Zhu Shao-Mi, Zhuang Jing, Liang Xin, Zhao Qing-Mei
机构信息
Center of Reproductive Medicine / Hospital of Reproduction, Maternity and Childcare, School of Medical and Life Sciences, Chengdu University of Chinese Medicine, Chengdu, Sichuan 610041, China.
出版信息
Zhonghua Nan Ke Xue. 2022 Oct;28(10):867-872.
OBJECTIVE
To investigate the knockdown of the outer dense fiber protein 2 (ODF2) gene on the sperm motility and fertility of male mice.
METHODS
We constructed three knockdown vectors with the target gene ODF2 and one control vector without the target gene. After infecting ICR mice, we determined the vector with the best knockdown effect by RT-PCR and Western blot and reinfected the mice with it. Then we obtained and analyzed the sperm motility parameters, pathological changes of the testis issue, and the litter size of the mice with gene knockdown.
RESULTS
Compared with the normal controls, the mice infected with the vector with the best knockdown effect showed significantly decreased sperm motility parameters, pathomorphological abnormalities of the testis, and a reduced litter size (10.86 ± 1.28 vs 12.72 ± 2.05, P = 0.001).
CONCLUSION
Decreased expression of the ODF2 gene deceases sperm motility parameters, impairs the morphology of the testis and affects the fertility of male mice.
目的
研究外致密纤维蛋白2（ODF2）基因敲低对雄性小鼠精子活力和生育能力的影响。
方法
构建3个针对目标基因ODF2的敲低载体和1个无目标基因的对照载体。感染ICR小鼠后，通过RT-PCR和蛋白质免疫印迹法确定敲低效果最佳的载体，并对小鼠进行再次感染。然后获取并分析基因敲低小鼠的精子活力参数、睾丸组织病理变化及产仔数。
结果
与正常对照组相比，感染敲低效果最佳载体的小鼠精子活力参数显著降低，睾丸出现病理形态学异常，产仔数减少（10.86±1.28 vs 12.72±2.05，P = 0.001）。
结论
ODF2基因表达降低会导致精子活力参数下降，损害睾丸形态并影响雄性小鼠的生育能力。
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