Food and Drug Department, University of Parma, Parma, Italy.
Risk Analysis and Genomic Epidemiology Unit, Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia-Romagna, Parma, Italy.
Int J Antimicrob Agents. 2024 Jan;63(1):107001. doi: 10.1016/j.ijantimicag.2023.107001. Epub 2023 Oct 13.
The aim of the project was to develop and characterise powders containing a probiotic (Lactiplantibacillus plantarum [Lpb. plantarum], Lacticaseibacillus rhamnosus, or Lactobacillus acidophilus) to be administered to the lung for the containment of pathogen growth in patients with lung infections.
The optimised spray drying process for the powder manufacturing was able to preserve viability of the bacteria, which decreased of only one log unit and was maintained up to 30 days.
Probiotic powders showed a high respirability (42%-50% of particles had a size < 5 µm) suitable for lung deposition and were proven safe on A549 and Calu-3 cells up to a concentration of 10 colony-forming units/mL. The Lpb. plantarum adhesion to both cell lines tested was at least 10%. Surprisingly, Lpb. plantarum powder was bactericidal at a concentration of 10 colony-forming units/mL on P. aeruginosa, whereas the other two strains were bacteriostatic.
This work represents a promising starting point to consider a probiotic inhalation powder a value in keeping the growth of pathogenic microflora in check during the antibiotic inhalation therapy suspension in cystic fibrosis treatment regimen. This approach could also be advantageous for interfering competitively with pathogenic bacteria and promoting the restoration of the healthy microbiota.
该项目旨在开发并表征含有益生菌（植物乳杆菌[Lpb. plantarum]、鼠李糖乳杆菌或嗜酸乳杆菌）的粉末，将其施用于肺部，以抑制肺部感染患者病原体的生长。
粉末制造的优化喷雾干燥工艺能够保持细菌的活力，细菌的活力仅下降了一个对数单位，并能维持 30 天。
益生菌粉末具有较高的可呼吸性（42%-50%的颗粒大小<5 µm），适合肺部沉积，并在 A549 和 Calu-3 细胞上证明在 10 菌落形成单位/mL 的浓度下是安全的。Lpb. plantarum 对两种测试细胞系的黏附率至少为 10%。令人惊讶的是，Lpb. plantarum 粉末在 10 菌落形成单位/mL 的浓度下对铜绿假单胞菌具有杀菌作用，而其他两种菌株则具有抑菌作用。
这项工作为考虑将益生菌吸入粉末作为在囊性纤维化治疗方案中抗生素吸入治疗暂停期间抑制病原微生物生长的一种有价值的方法提供了一个有希望的起点。这种方法还可能有利于与病原菌竞争，并促进健康微生物群的恢复。
