Byun Alex Seungyeon, Vitetta Luis, Chan Hak-Kim, Kwok Philip Chi Lip
Faculty of Medicine and Health, School of Pharmacy, University of Sydney, Sydney, NSW 2006, Australia.
Pharmaceutics. 2024 Oct 14;16(10):1326. doi: 10.3390/pharmaceutics16101326.
The use of probiotic bacteria to improve lung health has been gaining interest. Although the oral delivery of probiotics and their effects are well documented, there is currently limited knowledge on the respiratory delivery of probiotics.
This study aimed to investigate whether nebulisation is suitable for delivering GG (LGG) into the lungs for the potential treatment of bacterial pulmonary infections.
It compared the dose output and aerosol performance of a vibrating-mesh nebuliser (VMN) and a jet nebuliser (JN) in nebulising LGG suspended in de Man Rogosa Sharpe (MRS) broth, phosphate-buffered saline (PBS), or normal saline (0.9% / sodium chloride in water).
The VMN consistently produced a higher output than the JN for all liquid media, indicating that VMN was more efficient. The fine-particle fractions of both nebulisers were comparable for a given medium. The highest fine-particle fraction was achieved with LGG suspended in MRS broth for both nebulisers (20.5 ± 2.8% for VMN; 18.7 ± 3.4% for JN). This suggests that the aerosol performance of nebulised probiotics may depend on the medium in which the probiotic bacteria were suspended.
Therefore, this study demonstrated that the nebulisation efficiency of LGG depended on the nebuliser type and liquid medium of the probiotic suspension.
使用益生菌改善肺部健康已引起关注。尽管益生菌的口服给药及其效果已有充分记录，但目前关于益生菌经呼吸道给药的知识有限。
本研究旨在调查雾化是否适合将鼠李糖乳杆菌（LGG）输送至肺部以潜在治疗细菌性肺部感染。
比较了振动网孔雾化器（VMN）和喷射雾化器（JN）在雾化悬浮于德氏改良罗氏培养基（MRS肉汤）、磷酸盐缓冲盐水（PBS）或生理盐水（0.9%/水中氯化钠）中的LGG时的剂量输出和气溶胶性能。
对于所有液体介质，VMN始终比JN产生更高的输出，表明VMN效率更高。对于给定介质，两种雾化器的细颗粒部分相当。两种雾化器在MRS肉汤中悬浮的LGG时均达到最高细颗粒部分（VMN为20.5±2.8%；JN为18.7±3.4%）。这表明雾化益生菌的气溶胶性能可能取决于益生菌悬浮的介质。
因此，本研究表明LGG的雾化效率取决于雾化器类型和益生菌悬浮液的液体介质。
