Key Laboratory for Green Organic Synthesis and Application of Hunan Province, Key Laboratory of Environmentally Friendly Chemistry and Application of Ministry of Education, College of Chemistry, Xiangtan University, Xiangtan, 411105, PR China.
Henan Key Laboratory of Organic Functional Molecule and Drug Innovation, Collaborative Innovation Center of Henan Province for Green Manufacturing of Fine Chemicals, Key Laboratory of Green Chemical Media and Reactions, Ministry of Education, School of Chemistry and Chemical Engineering, Henan Normal University, Xinxiang, 453007, PR China.
Analyst. 2023 Nov 6;148(22):5724-5730. doi: 10.1039/d3an01442h.
Although hydrogen sulfide (HS) is a well-known toxic gas, its vital role as a gas transmitter in various physiological and pathological processes of living systems cannot be ignored. Relevant investigations indicate that endogenous HS is involved in the development of ulcerative colitis pathology and is overexpressed in ulcerative colitis, and hence can be considered as an ulcerative colitis biomarker. Herein, an isophorone-xanthene-based NIR fluorescent probe (IX-HS) was constructed to image HS. Owing to its large conjugated structure, the probe exhibits a near-infrared emission wavelength of 770 nm with a large Stokes shift (186 nm). Moreover, IX-HS has excellent selectivity for the detection of HS without interference from other analytes including thiols. In addition, the probe has been successfully applied not only in fluorescence imaging of endogenous and exogenous HS in living cells, but also in imaging of HS in normal and ulcerative colitis mice. Encouraged by the eminent performance, IX-HS is expected to be a potent "assistant" for the diagnosis of ulcerative colitis.
虽然硫化氢 (HS) 是一种众所周知的有毒气体，但它作为一种气体递质在生命系统的各种生理和病理过程中所发挥的重要作用不容忽视。相关研究表明，内源性 HS 参与溃疡性结肠炎的发病机制，并在溃疡性结肠炎中过度表达，因此可以被视为溃疡性结肠炎的生物标志物。在此，我们构建了一种基于异佛尔酮-呫吨的近红外荧光探针 (IX-HS) 来对 HS 进行成像。由于其较大的共轭结构，探针表现出近红外发射波长为 770nm，Stokes 位移较大（186nm）。此外，IX-HS 对 HS 的检测具有优异的选择性，不受包括硫醇在内的其他分析物的干扰。此外，该探针已成功应用于活细胞内源性和外源性 HS 的荧光成像，以及正常和溃疡性结肠炎小鼠 HS 的成像。鉴于其优异的性能，IX-HS 有望成为溃疡性结肠炎诊断的有力“助手”。
