Department of Urology & Nephrology, The First Affiliated Hospital of Ningbo University, 59 Liuting Street, Ningbo, Zhejiang, 315010, China.
College of Material Chemistry and Chemical Engineering, Key Laboratory of Organosilicon Chemistry and Material Technology, Ministry of Education, Key Laboratory of Organosilicon Material Technology of Zhejiang Province, Department Hangzhou Normal University, Hangzhou, Zhejiang, 311121, China.
Adv Sci (Weinh). 2023 Nov;10(32):e2303159. doi: 10.1002/advs.202303159. Epub 2023 Oct 15.
Castration-resistant prostate cancer (PCa) causes severe bone metastasis (BM), which significantly increases mortality in men with PCa. Imaging tests and radiometric scanning require long analysis times, expensive equipment, specialized personnel, and a slow turnaround. New visualization technologies are expected to solve the above problems. Nonetheless, existing visualization techniques barely meet the urgency for precise diagnosis because the human eyes cannot recognize and capture even slight variations in visual information. By using dye differentiated superposition enhancement colorimetric biosensors, an effective method to diagnose prostate cancer bone metastases (PCa-BM) with excellent accuracy for naked-eye quantitative detection of alkaline phosphatase (ALP) is developed. The biomarker ALP specific hydrolytic product ascorbic acid can be detected by rhodamine derivatives (Rd) as gold nanobipyramids (Au NBPs) are deposited and grown. Color-recombining enhancement effects between Rd and Au NBPs significantly improved abundance. The 150 U L threshold between normal and abnormal can be identified by color. And with color enhancement effect and double signal response, the ALP index is visually measured to diagnose PCa-BM and provide handy treatment recommendations. Additionally, the proposed colorimetric sensing strategy can be used to diagnose other diseases.
去势抵抗性前列腺癌 (PCa) 可导致严重的骨转移 (BM)，这会显著增加 PCa 男性的死亡率。影像学检查和放射性扫描需要长时间的分析，昂贵的设备、专业人员和缓慢的周转时间。人们期望新的可视化技术能够解决上述问题。然而，现有的可视化技术几乎无法满足精确诊断的紧迫性，因为人眼无法识别和捕捉到视觉信息中的细微变化。通过使用染料区分叠加增强比色生物传感器，开发了一种有效方法来诊断前列腺癌骨转移 (PCa-BM)，可通过肉眼对碱性磷酸酶 (ALP) 进行出色的定量检测。生物标志物 ALP 的特异性水解产物抗坏血酸可通过作为金纳米双吡咯烷 (Au NBPs) 沉积和生长的罗丹明衍生物 (Rd) 检测到。Rd 和 Au NBPs 之间的颜色再组合增强效果显著提高了丰度。可以通过颜色识别出 150 U/L 的正常和异常之间的阈值。通过颜色增强效果和双信号响应，对 ALP 指数进行直观测量，以诊断 PCa-BM 并提供便捷的治疗建议。此外，所提出的比色传感策略可用于诊断其他疾病。
