Ballester Roig Maria Neus, Mongrain Valérie
Department of Neuroscience, Université de Montréal, Montréal, QC, Canada.
Center for Advanced Research in Sleep Medicine, Recherche CIUSSS-NIM, Montréal, QC, Canada.
Sleep Adv. 2023 Sep 27;4(1):zpad037. doi: 10.1093/sleepadvances/zpad037. eCollection 2023.
We have recently demonstrated that the alkaloid rhynchophylline (RHY; purified from plants) induces sleep and modifies electrocorticographic (ECoG) activity throughout the 24-h day in a vigilance state-dependent manner in wild-type mice. We here asked whether this alkaloid impacts wake/sleep variables in the absence of the cell adhesion protein EPHA4, via ECoG recording in knockout (KO) mice submitted to the same RHY treatment contemporaneously to the wild-type mice (littermates). We uncover that RHY decreases time spent awake and increases time spent in slow wave sleep in KO mice and alters the 24-h time course of ECoG activity during wakefulness and sleep states. These observations are similar to the reported effects of RHY in wild-type littermate animals, which strongly supports that RHY-driven sleep alterations are not dependent on the presence of EPHA4.
我们最近证明，生物碱钩藤碱（RHY；从植物中纯化得到）能诱导野生型小鼠睡眠，并以与警觉状态相关的方式在24小时内改变其脑电活动（ECoG）。在此，我们通过对与野生型小鼠（同窝出生）同时接受相同RHY处理的基因敲除（KO）小鼠进行ECoG记录，来探究这种生物碱在缺乏细胞粘附蛋白EPHA4的情况下是否会影响清醒/睡眠变量。我们发现，RHY减少了KO小鼠的清醒时间，增加了其慢波睡眠的时间，并改变了清醒和睡眠状态下ECoG活动的24小时时间进程。这些观察结果与RHY在野生型同窝动物中的报道效应相似，这有力地支持了RHY驱动的睡眠改变不依赖于EPHA4的存在。
